| pubmed-article:10195883 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10195883 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:10195883 | lifeskim:mentions | umls-concept:C1506928 | lld:lifeskim |
| pubmed-article:10195883 | lifeskim:mentions | umls-concept:C1705438 | lld:lifeskim |
| pubmed-article:10195883 | lifeskim:mentions | umls-concept:C0007137 | lld:lifeskim |
| pubmed-article:10195883 | lifeskim:mentions | umls-concept:C0460004 | lld:lifeskim |
| pubmed-article:10195883 | lifeskim:mentions | umls-concept:C0151686 | lld:lifeskim |
| pubmed-article:10195883 | lifeskim:mentions | umls-concept:C1326205 | lld:lifeskim |
| pubmed-article:10195883 | lifeskim:mentions | umls-concept:C1518174 | lld:lifeskim |
| pubmed-article:10195883 | lifeskim:mentions | umls-concept:C1274040 | lld:lifeskim |
| pubmed-article:10195883 | lifeskim:mentions | umls-concept:C1517499 | lld:lifeskim |
| pubmed-article:10195883 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10195883 | pubmed:dateCreated | 1999-6-7 | lld:pubmed |
| pubmed-article:10195883 | pubmed:abstractText | E2F-1, a transcription factor by discovery, is thought to play a crucial role in regulating G1/S cell cycle progression. Its activity is modulated by complex formation with the retinoblastoma protein and related proteins. Overexpression of E2F-1 has been shown to induce apoptosis in quiescent fibroblasts. We constructed a recombinant E2F-1 adenovirus to test whether an overexpression of E2F-1 in head and neck squamous cell carcinoma cell lines would also induce apoptosis. Two cell lines, Tu-138 and Tu-167, were chosen for use in this study. Both cell lines harbor p53 mutations but express different levels of the retinoblastoma protein. Upon E2F-1 adenovirus infection, both cell lines expressed elevated levels of E2F-1 protein and then activated a pRb-chloramphenicol acetyltransferase reporter construct containing an E2F-1 binding motif. In vitro growth assay demonstrated that growth suppression by the E2F-1 protein was effective on both cell lines. Results from DNA fragmentation and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end-labeling analyses indicated apoptosis induction in cells infected with AdCMV-E2F-1. Moreover, ex vivo experiments in nude mice showed total suppression of tumor growth at sites that received cells infected AdCMV-E2F-1. An in vivo analysis of apoptosis using in situ end-labeling further demonstrated the induction of apoptosis by AdCMV-E2F-1 in tumor-bearing animals. These data indicate that overexpression of E2F-1 via an adenoviral vector suppresses in vitro and in vivo growth of head and neck squamous carcinoma cell lines through induction of apoptosis. | lld:pubmed |
| pubmed-article:10195883 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10195883 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10195883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195883 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10195883 | pubmed:issn | 0929-1903 | lld:pubmed |
| pubmed-article:10195883 | pubmed:author | pubmed-author:WangMM | lld:pubmed |
| pubmed-article:10195883 | pubmed:author | pubmed-author:DAWH THT | lld:pubmed |
| pubmed-article:10195883 | pubmed:author | pubmed-author:ClaymanG LGL | lld:pubmed |
| pubmed-article:10195883 | pubmed:author | pubmed-author:El-NaggarA... | lld:pubmed |
| pubmed-article:10195883 | pubmed:author | pubmed-author:HendersonYY | lld:pubmed |
| pubmed-article:10195883 | pubmed:author | pubmed-author:SteckK DKD | lld:pubmed |
| pubmed-article:10195883 | pubmed:author | pubmed-author:BreauR LRL | lld:pubmed |
| pubmed-article:10195883 | pubmed:author | pubmed-author:SicardM WMW | lld:pubmed |
| pubmed-article:10195883 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10195883 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:10195883 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10195883 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10195883 | pubmed:pagination | 163-71 | lld:pubmed |
| pubmed-article:10195883 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:10195883 | pubmed:articleTitle | Apoptosis induction by E2F-1 via adenoviral-mediated gene transfer results in growth suppression of head and neck squamous cell carcinoma cell lines. | lld:pubmed |
| pubmed-article:10195883 | pubmed:affiliation | Department of Head and Neck Surgery, University of Texas M.D. Anderson Cancer Center, Houston 77303, USA. tjlui@notes.mdacc.tmc.edu | lld:pubmed |
| pubmed-article:10195883 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10195883 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10195883 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10195883 | lld:pubmed |
