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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-6-7
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pubmed:abstractText |
E2F-1, a transcription factor by discovery, is thought to play a crucial role in regulating G1/S cell cycle progression. Its activity is modulated by complex formation with the retinoblastoma protein and related proteins. Overexpression of E2F-1 has been shown to induce apoptosis in quiescent fibroblasts. We constructed a recombinant E2F-1 adenovirus to test whether an overexpression of E2F-1 in head and neck squamous cell carcinoma cell lines would also induce apoptosis. Two cell lines, Tu-138 and Tu-167, were chosen for use in this study. Both cell lines harbor p53 mutations but express different levels of the retinoblastoma protein. Upon E2F-1 adenovirus infection, both cell lines expressed elevated levels of E2F-1 protein and then activated a pRb-chloramphenicol acetyltransferase reporter construct containing an E2F-1 binding motif. In vitro growth assay demonstrated that growth suppression by the E2F-1 protein was effective on both cell lines. Results from DNA fragmentation and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end-labeling analyses indicated apoptosis induction in cells infected with AdCMV-E2F-1. Moreover, ex vivo experiments in nude mice showed total suppression of tumor growth at sites that received cells infected AdCMV-E2F-1. An in vivo analysis of apoptosis using in situ end-labeling further demonstrated the induction of apoptosis by AdCMV-E2F-1 in tumor-bearing animals. These data indicate that overexpression of E2F-1 via an adenoviral vector suppresses in vitro and in vivo growth of head and neck squamous carcinoma cell lines through induction of apoptosis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arid4a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/E2F Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/E2F1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/E2F1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/E2f1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma-Binding Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor DP1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:issn |
0929-1903
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
163-71
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10195883-Adenoviridae,
pubmed-meshheading:10195883-Animals,
pubmed-meshheading:10195883-Apoptosis,
pubmed-meshheading:10195883-Blotting, Western,
pubmed-meshheading:10195883-Carcinoma, Squamous Cell,
pubmed-meshheading:10195883-Carrier Proteins,
pubmed-meshheading:10195883-Cell Cycle,
pubmed-meshheading:10195883-Cell Cycle Proteins,
pubmed-meshheading:10195883-Cell Division,
pubmed-meshheading:10195883-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:10195883-DNA Fragmentation,
pubmed-meshheading:10195883-DNA-Binding Proteins,
pubmed-meshheading:10195883-E2F Transcription Factors,
pubmed-meshheading:10195883-E2F1 Transcription Factor,
pubmed-meshheading:10195883-Head and Neck Neoplasms,
pubmed-meshheading:10195883-Humans,
pubmed-meshheading:10195883-In Situ Nick-End Labeling,
pubmed-meshheading:10195883-Mice,
pubmed-meshheading:10195883-Mice, Nude,
pubmed-meshheading:10195883-Neoplasms, Experimental,
pubmed-meshheading:10195883-Retinoblastoma-Binding Protein 1,
pubmed-meshheading:10195883-Time Factors,
pubmed-meshheading:10195883-Transcription Factor DP1,
pubmed-meshheading:10195883-Transcription Factors,
pubmed-meshheading:10195883-Tumor Cells, Cultured
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pubmed:articleTitle |
Apoptosis induction by E2F-1 via adenoviral-mediated gene transfer results in growth suppression of head and neck squamous cell carcinoma cell lines.
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pubmed:affiliation |
Department of Head and Neck Surgery, University of Texas M.D. Anderson Cancer Center, Houston 77303, USA. tjlui@notes.mdacc.tmc.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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