Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1999-7-6
pubmed:abstractText
The luteinizing hormone receptor (LHR) is alternatively spliced. It is not known if the alternatively spliced mRNAs are translated in vivo, or indeed if they have any vital role to play. The B splice form has been detected in every species examined, and it encodes a putative protein with a high affinity LH/CG binding domain but no trans-membrane or intra-cellular domains. We raised antisera that recognize the putative protein of the B form, and the closely related G form, and showed that the B form mRNA is translated in the ovine ovary, but not kidney or liver. It localized to the luteal cytosolic and microsomal fractions and the levels declined during regression induced by treatment with prostaglandin F2alpha. We examined alternative splicing by RNase protection analyses and RT-PCR analyses of healthy pre-ovulatory follicles, atretic or steroidogenically-inactive follicles, and of newly formed, mid-luteal and regressing corpora lutea. There was approximately 5-fold more B form mRNA than A form. Thus we have evidence that the LHR B form is translated in vivo, but no evidence that alternative splicing of the LHR mRNA is differentially regulated, throughout the oestrous cycle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
147
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
113-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10195698-Alternative Splicing, pubmed-meshheading:10195698-Animals, pubmed-meshheading:10195698-Corpus Luteum, pubmed-meshheading:10195698-Cytosol, pubmed-meshheading:10195698-Dinoprost, pubmed-meshheading:10195698-Estrus, pubmed-meshheading:10195698-Female, pubmed-meshheading:10195698-Gene Expression, pubmed-meshheading:10195698-Goats, pubmed-meshheading:10195698-Luteolysis, pubmed-meshheading:10195698-Microsomes, pubmed-meshheading:10195698-Mitochondria, pubmed-meshheading:10195698-Molecular Weight, pubmed-meshheading:10195698-Organ Size, pubmed-meshheading:10195698-Organ Specificity, pubmed-meshheading:10195698-Ovarian Follicle, pubmed-meshheading:10195698-Ovary, pubmed-meshheading:10195698-Protein Biosynthesis, pubmed-meshheading:10195698-Protein Isoforms, pubmed-meshheading:10195698-RNA, Messenger, pubmed-meshheading:10195698-Receptors, LH
pubmed:year
1999
pubmed:articleTitle
Characterization of the translated products of the alternatively spliced luteinizing hormone receptor in the ovine ovary throughout the oestrous cycle.
pubmed:affiliation
Department of Medicine, Flinders University of South Australia, Bedford Park, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't