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| pubmed-article:10194448 | lifeskim:mentions | umls-concept:C0017350 | lld:lifeskim |
| pubmed-article:10194448 | lifeskim:mentions | umls-concept:C1441547 | lld:lifeskim |
| pubmed-article:10194448 | lifeskim:mentions | umls-concept:C1704970 | lld:lifeskim |
| pubmed-article:10194448 | lifeskim:mentions | umls-concept:C1513380 | lld:lifeskim |
| pubmed-article:10194448 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:10194448 | lifeskim:mentions | umls-concept:C0205349 | lld:lifeskim |
| pubmed-article:10194448 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:10194448 | pubmed:dateCreated | 1999-5-3 | lld:pubmed |
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| pubmed-article:10194448 | pubmed:abstractText | T-cell-rich B-cell lymphoma (TCRBCL) belongs to the group of diffuse large cell lymphomas (DLL). It is characterized by a small number of tumor B cells among a major population of nonmalignant polyclonal T cells. To identify the developmental stage of the tumor progenitor cells, we micromanipulated the putative neoplastic large CD20(+) cells from TCRBCLs and amplified and sequenced immunoglobulin (Ig) V gene rearrangements from individual cells. In six cases, clonal Ig heavy, as well as light chain, gene rearrangements were amplified from the isolated B cells. All six cases harbored somatically mutated V gene rearrangements with an average mutation frequency of 15.5% for heavy (VH) and 5.9% for light (VL) chains and intraclonal diversity based on somatic mutation. These findings identify germinal center (GC) B cells as the precursors of the transformed B cells in TCRBCL. The study also exemplifies various means how Ig gene rearrangements can be modified by GC B cells or their malignant counterparts in TCRBCL: In one case, the tumor precursor may have switched from kappa to lambda light chain expression after acquiring a crippling mutation within the initially functional kappa light chain gene. In another case, the tumor cells harbor two in-frame VH gene rearrangements, one of which was rendered nonfunctional by somatic mutation. Either the tumor cell precursor entered the GC with two potentially functional in-frame rearrangements or the second VHDHJH rearrangement occurred in the GC after the initial in-frame rearrangement was inactivated by somatic mutation. Finally, in each of the six cases, at least one cell contained two (or more) copies of a clonal Ig gene rearrangement with sequence variations between these copies. The presence of sequence variants for V region genes within single B cells has so far not been observed in any other normal or transformed B lymphocyte. Fluorescence in situ hybridization (FISH) points to a generalized polyploidy of the tumor cells. | lld:pubmed |
| pubmed-article:10194448 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10194448 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10194448 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:10194448 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10194448 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:10194448 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:10194448 | pubmed:author | pubmed-author:RajewskyKK | lld:pubmed |
| pubmed-article:10194448 | pubmed:author | pubmed-author:SiebertRR | lld:pubmed |
| pubmed-article:10194448 | pubmed:author | pubmed-author:KüppersRR | lld:pubmed |
| pubmed-article:10194448 | pubmed:author | pubmed-author:HansmannM LML | lld:pubmed |
| pubmed-article:10194448 | pubmed:author | pubmed-author:Schlegelberge... | lld:pubmed |
| pubmed-article:10194448 | pubmed:author | pubmed-author:StricklerJ... | lld:pubmed |
| pubmed-article:10194448 | pubmed:author | pubmed-author:BräuningerAA | lld:pubmed |
| pubmed-article:10194448 | pubmed:author | pubmed-author:SpiekerTT | lld:pubmed |
| pubmed-article:10194448 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10194448 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:10194448 | pubmed:volume | 93 | lld:pubmed |
| pubmed-article:10194448 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10194448 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10194448 | pubmed:pagination | 2679-87 | lld:pubmed |
| pubmed-article:10194448 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:10194448 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10194448 | pubmed:articleTitle | Molecular analysis of single B cells from T-cell-rich B-cell lymphoma shows the derivation of the tumor cells from mutating germinal center B cells and exemplifies means by which immunoglobulin genes are modified in germinal center B cells. | lld:pubmed |
| pubmed-article:10194448 | pubmed:affiliation | Department of Pathology, University of Frankfurt, Germany. Braeununger@em.uni-frankfurt.de | lld:pubmed |
| pubmed-article:10194448 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10194448 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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