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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1999-5-3
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130895,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130896,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130897,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130898,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130899,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130900,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130901,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130902,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130903,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130904,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130905,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130906,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130907,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130908,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130909,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130910,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130911,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130912,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130913,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ130914
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pubmed:abstractText |
T-cell-rich B-cell lymphoma (TCRBCL) belongs to the group of diffuse large cell lymphomas (DLL). It is characterized by a small number of tumor B cells among a major population of nonmalignant polyclonal T cells. To identify the developmental stage of the tumor progenitor cells, we micromanipulated the putative neoplastic large CD20(+) cells from TCRBCLs and amplified and sequenced immunoglobulin (Ig) V gene rearrangements from individual cells. In six cases, clonal Ig heavy, as well as light chain, gene rearrangements were amplified from the isolated B cells. All six cases harbored somatically mutated V gene rearrangements with an average mutation frequency of 15.5% for heavy (VH) and 5.9% for light (VL) chains and intraclonal diversity based on somatic mutation. These findings identify germinal center (GC) B cells as the precursors of the transformed B cells in TCRBCL. The study also exemplifies various means how Ig gene rearrangements can be modified by GC B cells or their malignant counterparts in TCRBCL: In one case, the tumor precursor may have switched from kappa to lambda light chain expression after acquiring a crippling mutation within the initially functional kappa light chain gene. In another case, the tumor cells harbor two in-frame VH gene rearrangements, one of which was rendered nonfunctional by somatic mutation. Either the tumor cell precursor entered the GC with two potentially functional in-frame rearrangements or the second VHDHJH rearrangement occurred in the GC after the initial in-frame rearrangement was inactivated by somatic mutation. Finally, in each of the six cases, at least one cell contained two (or more) copies of a clonal Ig gene rearrangement with sequence variations between these copies. The presence of sequence variants for V region genes within single B cells has so far not been observed in any other normal or transformed B lymphocyte. Fluorescence in situ hybridization (FISH) points to a generalized polyploidy of the tumor cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2679-87
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10194448-Adult,
pubmed-meshheading:10194448-Aged,
pubmed-meshheading:10194448-Aged, 80 and over,
pubmed-meshheading:10194448-Antigens, CD,
pubmed-meshheading:10194448-Antigens, CD20,
pubmed-meshheading:10194448-Female,
pubmed-meshheading:10194448-Gene Rearrangement,
pubmed-meshheading:10194448-Genes, Immunoglobulin,
pubmed-meshheading:10194448-Genetic Variation,
pubmed-meshheading:10194448-Humans,
pubmed-meshheading:10194448-Immunoglobulin Heavy Chains,
pubmed-meshheading:10194448-Immunoglobulin Light Chains,
pubmed-meshheading:10194448-Immunoglobulin kappa-Chains,
pubmed-meshheading:10194448-Immunoglobulin lambda-Chains,
pubmed-meshheading:10194448-Lymph Nodes,
pubmed-meshheading:10194448-Lymphoma, B-Cell,
pubmed-meshheading:10194448-Male,
pubmed-meshheading:10194448-Middle Aged,
pubmed-meshheading:10194448-Polymerase Chain Reaction,
pubmed-meshheading:10194448-T-Lymphocytes
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pubmed:year |
1999
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pubmed:articleTitle |
Molecular analysis of single B cells from T-cell-rich B-cell lymphoma shows the derivation of the tumor cells from mutating germinal center B cells and exemplifies means by which immunoglobulin genes are modified in germinal center B cells.
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pubmed:affiliation |
Department of Pathology, University of Frankfurt, Germany. Braeununger@em.uni-frankfurt.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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