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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1999-6-7
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pubmed:abstractText |
Recombinant reassortment technology was used to prepare H5N1 influenza vaccine strains containing a modified hemagglutinin (HA) gene and neuraminidase gene from the A/Hong Kong/156/97 and A/Hong Kong/483/97 isolates and the internal genes from the attenuated cold-adapted A/Ann Arbor/6/60 influenza virus strain. The HA cleavage site (HA1/HA2) of each H5N1 isolate was modified to resemble that of "low-pathogenic" avian strains. Five of 6 basic amino acids at the cleavage site were deleted, and a threonine was added upstream of the remaining arginine. The H5 HA cleavage site modification resulted in the expected trypsin-dependent phenotype without altering the antigenic character of the H5 HA molecule. The temperature-sensitive and cold-adapted phenotype of the attenuated parent virus was maintained in the recombinant strains, and they grew to 108.5-9.4 EID50/mL in eggs. Both H5N1 vaccine virus strains were safe and immunogenic in ferrets and protected chickens against wild-type H5N1 virus challenge.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Hemagglutinin Glycoproteins...,
http://linkedlifedata.com/resource/pubmed/chemical/Influenza Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Neuraminidase,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Attenuated,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1899
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
179
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1132-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10191214-Amino Acid Sequence,
pubmed-meshheading:10191214-Animals,
pubmed-meshheading:10191214-Antibodies, Viral,
pubmed-meshheading:10191214-Antigens, Viral,
pubmed-meshheading:10191214-Chickens,
pubmed-meshheading:10191214-Drug Design,
pubmed-meshheading:10191214-Ferrets,
pubmed-meshheading:10191214-Genes, Viral,
pubmed-meshheading:10191214-Hemagglutinin Glycoproteins, Influenza Virus,
pubmed-meshheading:10191214-Humans,
pubmed-meshheading:10191214-Influenza, Human,
pubmed-meshheading:10191214-Influenza A Virus, H5N1 Subtype,
pubmed-meshheading:10191214-Influenza A virus,
pubmed-meshheading:10191214-Influenza Vaccines,
pubmed-meshheading:10191214-Molecular Sequence Data,
pubmed-meshheading:10191214-Neuraminidase,
pubmed-meshheading:10191214-Vaccines, Attenuated,
pubmed-meshheading:10191214-Vaccines, Synthetic,
pubmed-meshheading:10191214-Viral Plaque Assay
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pubmed:year |
1999
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pubmed:articleTitle |
Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses.
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pubmed:affiliation |
Aviron, Mountain View, California Veterinary Diagnostic Laboratory CA System, Davis, CA, USA. sli@aviron.com
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pubmed:publicationType |
Journal Article
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