Source:http://linkedlifedata.com/resource/pubmed/id/10191045
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1999-5-11
|
| pubmed:abstractText |
The Cre/loxP site-specific recombination system combined with embryonic stem cell-mediated technologies has greatly expanded our capability to address normal and disease development in mammals using genetic approaches. The success of this emerging technology hinges on the production of Cre-expressing transgenic lines that provide cell type-, tissue-, or developmental stage-specific recombination between loxP sites placed in the genome. Here we describe and characterize the production of a double-reporter mouse line that provides a convenient and reliable readout of Cre recombinase activity. Throughout all embryonic and adult stages, the transgenic animal expresses the lacZ reporter gene before Cre-mediated excision occurs. Cre excision, however, removes the lacZ gene, allowing expression of the second reporter, the human alkaline phosphatase gene. This double-reporter transgenic line is able to indicate the occurrence of Cre excision in an extremely widespread manner from early embryonic to adult lineages. It will be a valuable reagent for the increasing number of investigators taking advantage of the powerful tools provided by the Cre/loxP site-specific recombinase system.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0012-1606
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1999 Academic Press.
|
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
208
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
281-92
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10191045-Alkaline Phosphatase,
pubmed-meshheading:10191045-Animals,
pubmed-meshheading:10191045-Cell Aggregation,
pubmed-meshheading:10191045-Embryo, Mammalian,
pubmed-meshheading:10191045-Gene Expression,
pubmed-meshheading:10191045-Genes, Reporter,
pubmed-meshheading:10191045-Genetic Vectors,
pubmed-meshheading:10191045-Genotype,
pubmed-meshheading:10191045-Histocytochemistry,
pubmed-meshheading:10191045-Integrases,
pubmed-meshheading:10191045-Lac Operon,
pubmed-meshheading:10191045-Mice,
pubmed-meshheading:10191045-Mice, Transgenic,
pubmed-meshheading:10191045-Ploidies,
pubmed-meshheading:10191045-Recombination, Genetic,
pubmed-meshheading:10191045-Sequence Deletion,
pubmed-meshheading:10191045-Stem Cells,
pubmed-meshheading:10191045-Tissue Distribution,
pubmed-meshheading:10191045-Transgenes,
pubmed-meshheading:10191045-Viral Proteins
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Z/AP, a double reporter for cre-mediated recombination.
|
| pubmed:affiliation |
Cancer Research Division, Sunnybrook Health Science Centre, 2075 Bayview Avenue, Toronto, Ontario, M4N 3M5, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|