Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-5-6
pubmed:abstractText
Receptor recycling involves two endosome populations, peripheral early endosomes and perinuclear recycling endosomes. In polarized epithelial cells, either or both populations must be able to sort apical from basolateral proteins, returning each to its appropriate plasma membrane domain. However, neither the roles of early versus recycling endosomes in polarity nor their relationship to each other has been quantitatively evaluated. Using a combined morphological, biochemical, and kinetic approach, we found these two endosome populations to represent physically and functionally distinct compartments. Early and recycling endosomes were resolved on Optiprep gradients and shown to be differentially associated with rab4, rab11, and transferrin receptor; rab4 was enriched on early endosomes and at least partially depleted from recycling endosomes, with the opposite being true for rab11 and transferrin receptor. The two populations were also pharmacologically distinct, with AlF4 selectively blocking export of transferrin receptor from recycling endosomes to the basolateral plasma membrane. We applied these observations to a detailed kinetic analysis of transferrin and dimeric IgA recycling and transcytosis. The data from these experiments permitted the construction of a testable, mathematical model which enabled a dissection of the roles of early and recycling endosomes in polarized receptor transport. Contrary to expectations, the majority (>65%) of recycling to the basolateral surface is likely to occur from early endosomes, but with relatively little sorting of apical from basolateral proteins. Instead, more complete segregation of basolateral receptors from receptors intended for transcytosis occurred upon delivery to recycling endosomes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-1356629, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-1419044, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-1547485, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-1691196, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-1934063, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-2110383, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-2209555, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-2380185, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-2558276, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-2760105, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-2821012, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-3175765, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-3315805, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-3345561, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-6204769, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-6279628, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-6289322, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-6309781, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-6309862, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-6322002, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-6324805, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-7328118, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-7495576, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-7599439, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-7790351, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-7860630, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-7905002, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-7961930, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-7983176, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-7986532, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-8138576, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-8311840, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-8376460, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-8402881, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-8486736, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-8509447, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-8790369, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-8858169, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-8922376, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-8970738, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-9177342, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-9236981, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-9452505, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-9490718, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-9553086, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-9566963, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-9624171, http://linkedlifedata.com/resource/pubmed/commentcorrection/10189373-9763422
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Aluminum Compounds, http://linkedlifedata.com/resource/pubmed/chemical/Fluorides, http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin A, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Polymeric Immunoglobulin, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transferrin, http://linkedlifedata.com/resource/pubmed/chemical/polymeric IgA, http://linkedlifedata.com/resource/pubmed/chemical/rab GTP-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/rab11 protein, http://linkedlifedata.com/resource/pubmed/chemical/rab4 GTP-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/tetrafluoroaluminate
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
145
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
123-39
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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