Linked Life Data

10189189

Source:http://linkedlifedata.com/resource/pubmed/id/10189189

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-5-28
pubmed:abstractText
Human (h) interleukin-10 (IL-10) exhibits a strong DNA and amino acid sequence homology to the Epstein-Barr virus (EBV) BCRF1 genome, viral (v) IL-10. We analyzed the production of IL-10 for EBV activation in B-cell lines. The latent EBV in Akata cells was activated by the cross-linking of surface immunoglobulin G (IgG) with anti-human IgG. The levels of IL-10(h+v) and vIL-10 in the culture fluids were measured by a specific enzyme-linked immunosorbent assay (ELISA). IL-10(h+v) was detected at the same time for EBV immediate early gene BZLF1 product ZEBRA and early gene BMRF1 product EA-D. This was more than 4 hours prior to the appearance of vIL-10, and late gene products gp 350/220 and viral capsid antigen. The induction of hIL-10 and vIL-10 mRNAs were detected in anti-IgG-treated Akata cells by reverse transcription-polymerase chain reaction. The induction of IL-10(h+v) and vIL-10 was inhibited with a tyrosine kinase inhibitor, herbimycin, or with an inhibitor of herpesvirus DNA polymerase, phosphonoacetic acid, or acyclovir. IL-10(h+v) and vIL-10 were also detected in the supernatants of Akata and Daudi but not Ramos cells infected with P3HR-1 EBV. These results show the IL-10 induction on EBV activation in EBV-carrying B-cell lines.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0882-8245
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
221-31
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Induction of interleukin-10 on activation of Epstein-Barr virus in EBV-infected B-cell lines.
pubmed:affiliation
Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't