| pubmed-article:10168562 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10168562 | lifeskim:mentions | umls-concept:C1527169 | lld:lifeskim |
| pubmed-article:10168562 | lifeskim:mentions | umls-concept:C0013018 | lld:lifeskim |
| pubmed-article:10168562 | lifeskim:mentions | umls-concept:C0079459 | lld:lifeskim |
| pubmed-article:10168562 | lifeskim:mentions | umls-concept:C1518999 | lld:lifeskim |
| pubmed-article:10168562 | lifeskim:mentions | umls-concept:C1516698 | lld:lifeskim |
| pubmed-article:10168562 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:10168562 | pubmed:dateCreated | 1997-8-19 | lld:pubmed |
| pubmed-article:10168562 | pubmed:abstractText | Peripheral blood stems cells (PBSCs) have been used in autologous transplantation as an alternative to bone marrow-derived cells. Recently, PBSCs have been collected from healthy donors after priming with G-CSF and used for allogeneic transplantation. We have a comparatively large experience with PBSC collection in autologous and allogeneic settings. The five cell separators we employ are: the CS3000 plus, AS 104, Excel, Cobe Spectra and MCS 3p. These machines appear to have different efficacies but no studies have been carried out on this topic. In a prospective study we have randomly assigned donors to different cell separators to evaluate their efficiency. Twenty-five donors underwent the procedure and 50 leukaphereses were carried out. Donors were given 5 micrograms kg-1 d-1 of recombinant human G-CSF for 3 days and 10 micrograms kg-1 d-1 for 4 days subcutaneously. Leukaphereses were performed on days 6 and 7 of G-CSF administration. The results of our study show that a total value of CD34+ cells ranging from 48.44 x 10(6) to 270.37 x 10(6) can be collected from donors with a white cell count ranging from 40.50 x 10(3) microL-1 to 51.34 x 10(3) microL-1 and mononuclear cells ranging from 16.42 to 20.37%. The Excel and the MCS 3p seem to differ from the other machines in terms of higher CD34+ cell collection efficiency. The Excel appears to be even more efficient than the MCS 3p, but this may not reflect reality because the Excel processes 12 L of blood while the MCS 3p employs an 8 L procedure. All the machines showed satisfactory results in terms of yield and quality of the harvests. | lld:pubmed |
| pubmed-article:10168562 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10168562 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10168562 | pubmed:citationSubset | T | lld:pubmed |
| pubmed-article:10168562 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10168562 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10168562 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10168562 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10168562 | pubmed:issn | 0955-3886 | lld:pubmed |
| pubmed-article:10168562 | pubmed:author | pubmed-author:ValbonesiMM | lld:pubmed |
| pubmed-article:10168562 | pubmed:author | pubmed-author:BenvenutoFF | lld:pubmed |
| pubmed-article:10168562 | pubmed:author | pubmed-author:FlorioGG | lld:pubmed |
| pubmed-article:10168562 | pubmed:author | pubmed-author:CarlierPP | lld:pubmed |
| pubmed-article:10168562 | pubmed:author | pubmed-author:PollicardoNN | lld:pubmed |
| pubmed-article:10168562 | pubmed:author | pubmed-author:FigariOO | lld:pubmed |
| pubmed-article:10168562 | pubmed:author | pubmed-author:PungolinoEE | lld:pubmed |
| pubmed-article:10168562 | pubmed:author | pubmed-author:RuzzenentiM... | lld:pubmed |
| pubmed-article:10168562 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10168562 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:10168562 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10168562 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10168562 | pubmed:pagination | 619-27 | lld:pubmed |
| pubmed-article:10168562 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:10168562 | pubmed:meshHeading | pubmed-meshheading:10168562... | lld:pubmed |
| pubmed-article:10168562 | pubmed:meshHeading | pubmed-meshheading:10168562... | lld:pubmed |
| pubmed-article:10168562 | pubmed:meshHeading | pubmed-meshheading:10168562... | lld:pubmed |
| pubmed-article:10168562 | pubmed:meshHeading | pubmed-meshheading:10168562... | lld:pubmed |
| pubmed-article:10168562 | pubmed:meshHeading | pubmed-meshheading:10168562... | lld:pubmed |
| pubmed-article:10168562 | pubmed:meshHeading | pubmed-meshheading:10168562... | lld:pubmed |
| pubmed-article:10168562 | pubmed:meshHeading | pubmed-meshheading:10168562... | lld:pubmed |
| pubmed-article:10168562 | pubmed:meshHeading | pubmed-meshheading:10168562... | lld:pubmed |
| pubmed-article:10168562 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:10168562 | pubmed:articleTitle | PBSC collection from G-CSF primed donors. | lld:pubmed |
| pubmed-article:10168562 | pubmed:affiliation | Immunohematology Service S. Martino Hospital, Genoa, Italy. | lld:pubmed |
| pubmed-article:10168562 | pubmed:publicationType | Journal Article | lld:pubmed |
