Linked Life Data

1015031

Source:http://linkedlifedata.com/resource/pubmed/id/1015031

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1977-3-31
pubmed:abstractText
The endotoxin (lipopolysaccharide) preparations were extracted by the BOIVIN method from 10 strains of Erwinia herbicola isolated from the air of grain mills and from human and animal sources. It was found in assays for biological activity that these preparations had true endotoxic properties: lethality for mice, ability to produce primary inflammatory lesions in rabbit skin and ability to prepare rabbit skin for the local SHWARTZMAN reaction. Endotoxins obtained from five E. herbicola isolates were highly toxic and had mouse LD50 values ranging from 0.23 to 0.50 mg. The reparations derived from the remaining five strains were less potent with LD50 values ranging from 0.96 to 2.83 mg. The endotoxins of E. herbicola caused primary skin lesions (edema and/or erythema) in rabbits in the mean threshold doses (SLD50) of 1.33 to 5.94 mug and had the ability to prepare the rabbit skin for the local SHWARTZMAN reaction in the mean threshold does (SPD50) of 2.97 to 95.0 mug. The endotoxic properties of the E. herbicola preparations were similar to those of simultaneously tested enterobacterial lipopolysaccharides. The results of the mouse toxicity tests were positively correlated with those of the rabbit skin tests. In the additional tests the single preparations of E. herbicola showed two other endotoxic properties: ability to produce hemorrhagic lesions in rabbit skin after mixing with epinephrine and lethal effect on chick embryo. A preliminary chemical analysis of the trichloroacetic extracts of E. herbicola revealed low nitrogen and high carbohydrate contents as well as the presence of the common monosaccharides, reported in literature for endotoxins of various gram-negative bacteria. The significance of the presence of endotoxins in the ubiquitous E. herbicola rods is discussed, particularly with respect to occupational health hazard resulting from inhalation of vegetable dusts containing these organisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0300-9688
pubmed:author
pubmed:issnType
Print
pubmed:volume
236
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
487-508
pubmed:dateRevised
2009-6-5
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Studies on endotoxin of Erwinia herbicola and their biological activity.
pubmed:publicationType
Journal Article