Linked Life Data

10101681

Source:http://linkedlifedata.com/resource/pubmed/id/10101681

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-6-9
pubmed:abstractText
Renal cell carcinoma (RCC) is the most common renal neoplasm. Despite being infiltrated by tumour infiltrating lymphocytes (TIL), these TIL are unable to control tumour growth in vivo, suggesting that the cytotoxic capacity of TIL against RCC is impaired, or that the tumour cells are resistant to killing and therefore escape detection by the immune system. It is postulated that the expression of apoptotic regulatory molecules in RCC favours tumour cell survival. The present study has therefore determined the expression of Fas (APO-1/CD95), Fas ligand (Fas L) and bcl-2 in these tumours. The expression of Fas, Fas L and bcl-2 mRNA transcripts was determined in RCC, normal kidney and peripheral blood by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), following RNA extraction and cDNA synthesis from tissues and cell samples. Transcript levels were measured by densitometry after Southern blot hybridization of PCR products with internal radio-labelled oligonucleotide probes; a densitometry score was assigned to each hybridizing DNA band and expressed as a ratio of the glyceraldehyde-3-phosphate dehydrogenase content. In peripheral blood, the expression of Fas L and bcl-2 transcripts was similar between patients and normal healthy individuals; however, Fas transcript expression was significantly down-regulated in the patients' versus normal peripheral blood (P = 0.026). Most interestingly, significantly up-regulated Fas L expression was observed in RCC compared to normal kidney (P = 0.041). In contrast, bcl-2 transcripts were well represented in normal kidney but markedly decreased in RCC (P = 0.021). The expression of Fas transcripts in normal kidney and RCC was variable. These data demonstrate elevated expression of Fas L transcripts in RCC, but the functional relevance of this remains to be investigated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0818-9641
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10101681-Adult, pubmed-meshheading:10101681-Aged, pubmed-meshheading:10101681-Antigens, CD95, pubmed-meshheading:10101681-Base Sequence, pubmed-meshheading:10101681-Carcinoma, Renal Cell, pubmed-meshheading:10101681-Case-Control Studies, pubmed-meshheading:10101681-DNA Primers, pubmed-meshheading:10101681-Fas Ligand Protein, pubmed-meshheading:10101681-Female, pubmed-meshheading:10101681-Gene Expression, pubmed-meshheading:10101681-Genes, bcl-2, pubmed-meshheading:10101681-Humans, pubmed-meshheading:10101681-Kidney Neoplasms, pubmed-meshheading:10101681-Lymphocytes, Tumor-Infiltrating, pubmed-meshheading:10101681-Male, pubmed-meshheading:10101681-Membrane Glycoproteins, pubmed-meshheading:10101681-Middle Aged, pubmed-meshheading:10101681-RNA, Messenger, pubmed-meshheading:10101681-RNA, Neoplasm, pubmed-meshheading:10101681-T-Lymphocytes, Cytotoxic
pubmed:year
1999
pubmed:articleTitle
Expression of apoptotic regulatory molecules in renal cell carcinoma: elevated expression of Fas ligand.
pubmed:affiliation
Department of Renal Medicine, Princes Alexandra Hospital, Woolloongabba, Brisbane, Queensland, Australia. mdcolive@dingo.uq.edu.au
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't