Linked Life Data

10101240

Source:http://linkedlifedata.com/resource/pubmed/id/10101240

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-5-21
pubmed:abstractText
We have cloned the promoter regions of the genes for the mouse and human gamma2 subunits of the type A receptors for gamma-aminobutyric acid (GABA). For the mouse, the two major transcription start sites were at +1 (by definition) and +43, as established by rapid amplification of cDNA ends (RACE) and primer extension. This numbering places the start methionine at +297. There was no TATA or CCAAT box. Both mouse and human sequences have a candidate neuron-restrictive silencer element (NRSE) site in the first intron (+956 in mouse). We made assorted mouse-based promoter/reporter (luciferase) constructs starting from a core extending from -331 to +136, varying sizes at both ends, and including and excluding the putative NRSE and more proximal sequences. These were tested by transient transfection in several neuron-like and non-neuronal cell lines. Both proximal and distal downstream elements appeared to help direct expression to neuron-like cells, the NRSE in the intron, by repression in non-neurons, and a 24-bp portion of the 5' untranslated region starting at +113 (named GPE1) by preferentially promoting expression in neuron-like cells. Cotransfected human NRSF (transcription factor for NRSE) reduced reporter expression in neuron-like cells for constructs containing the NRSE in two locations. In gel mobility shift assays, the mouse gamma2 NRSE and a consensus NRSE both bound in vitro translated NRSF very similarly, and the NRSF gave the same major shifted band with the mouse gamma2 NRSE as was observed with nuclear extracts.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0169-328X
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Elsevier Science B.V.
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-47
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10101240-3T3 Cells, pubmed-meshheading:10101240-Animals, pubmed-meshheading:10101240-Base Sequence, pubmed-meshheading:10101240-Benzodiazepines, pubmed-meshheading:10101240-Brain Chemistry, pubmed-meshheading:10101240-Cloning, Molecular, pubmed-meshheading:10101240-DNA, Complementary, pubmed-meshheading:10101240-DNA Primers, pubmed-meshheading:10101240-Gene Expression Regulation, pubmed-meshheading:10101240-Genes, Reporter, pubmed-meshheading:10101240-HeLa Cells, pubmed-meshheading:10101240-Humans, pubmed-meshheading:10101240-Luciferases, pubmed-meshheading:10101240-Mice, pubmed-meshheading:10101240-Mice, Inbred C57BL, pubmed-meshheading:10101240-Molecular Sequence Data, pubmed-meshheading:10101240-Plasmids, pubmed-meshheading:10101240-Promoter Regions, Genetic, pubmed-meshheading:10101240-Protein Structure, Tertiary, pubmed-meshheading:10101240-Receptors, GABA-A, pubmed-meshheading:10101240-Sequence Homology, Amino Acid, pubmed-meshheading:10101240-Transcriptional Activation, pubmed-meshheading:10101240-Transfection, pubmed-meshheading:10101240-gamma-Aminobutyric Acid
pubmed:year
1999
pubmed:articleTitle
Transcriptional regulation of GABAA receptor gamma2 subunit gene.
pubmed:affiliation
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201-1559, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't