Linked Life Data

10101017

Source:http://linkedlifedata.com/resource/pubmed/id/10101017

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-5-13
pubmed:abstractText
The distribution and regulation of leukemia inhibitory factor (LIF) and its receptor (LIFR) in human lung tissue is unknown. We recently found that LIF was immunolocalized to several cell types in human airways, and that exogenous LIF modulated neural and contractile responses of explanted airways. The present study aimed to determine the cellular distribution and regulation of gene transcripts for LIF and LIFR in human lung, and measured the release of LIF in response to anti-immunoglobulin (Ig)E, interleukin (IL)-1beta, and IL-6. Exposure of human lung to IL-1beta (100 pg/ml) resulted in the rapid induction of LIF messenger RNA (mRNA) (1 h) and subsequent protein release (6 h). Similar results were observed when lung tissue was exposed to anti-IgE (6 U/ml). Gene transcripts for LIF were observed in nine pulmonary cell types, with the greatest expression occurring in fibroblasts. LIFR transcripts were also widely expressed in these cell types. In cultures of nontransformed epithelial cells, lung fibroblasts, and airway smooth-muscle cells, IL-1beta (100 pg/ml) induced the rapid accumulation of LIF mRNA and protein release, with fibroblasts liberating the greatest amount. IL-6 also induced the expression of LIF mRNA and release of LIF in airway smooth-muscle cells, whereas exogenous LIF itself had no effect. Expression of LIFR mRNA was not influenced by exposure to IL-1beta or LIF in any of the cell lines used. These results highlight the widespread distribution and rapid release of LIF in human lung tissue and, in conjunction with our previous report, suggest that this cytokine may play an important role in lung inflammatory processes and neuroimmune interactions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1044-1549
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
834-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10101017-Bronchi, pubmed-meshheading:10101017-Cells, Cultured, pubmed-meshheading:10101017-DNA Primers, pubmed-meshheading:10101017-Endothelium, Vascular, pubmed-meshheading:10101017-Epithelial Cells, pubmed-meshheading:10101017-Growth Inhibitors, pubmed-meshheading:10101017-Humans, pubmed-meshheading:10101017-Interleukin-1, pubmed-meshheading:10101017-Interleukin-6, pubmed-meshheading:10101017-Leukemia Inhibitory Factor, pubmed-meshheading:10101017-Leukemia Inhibitory Factor Receptor alpha Subunit, pubmed-meshheading:10101017-Lung, pubmed-meshheading:10101017-Lymphokines, pubmed-meshheading:10101017-Muscle, Smooth, Vascular, pubmed-meshheading:10101017-Pulmonary Artery, pubmed-meshheading:10101017-Receptors, Cytokine, pubmed-meshheading:10101017-Receptors, OSM-LIF, pubmed-meshheading:10101017-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10101017-Transcription, Genetic
pubmed:year
1999
pubmed:articleTitle
Leukemia inhibitory factor (LIF) and LIF receptor in human lung. Distribution and regulation of LIF release.
pubmed:affiliation
University of British Columbia Pulmonary Research Laboratory, St. Paul's Hospital, Vancouver, British Columbia, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't