| pubmed-article:10099510 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10099510 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:10099510 | lifeskim:mentions | umls-concept:C0376432 | lld:lifeskim |
| pubmed-article:10099510 | lifeskim:mentions | umls-concept:C0205332 | lld:lifeskim |
| pubmed-article:10099510 | lifeskim:mentions | umls-concept:C0032521 | lld:lifeskim |
| pubmed-article:10099510 | lifeskim:mentions | umls-concept:C0183683 | lld:lifeskim |
| pubmed-article:10099510 | lifeskim:mentions | umls-concept:C1707689 | lld:lifeskim |
| pubmed-article:10099510 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10099510 | pubmed:dateCreated | 1999-5-7 | lld:pubmed |
| pubmed-article:10099510 | pubmed:abstractText | Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4, 4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlactone functional groups. Optimization of hydrophilic/hydrophobic properties of the monolith affords a support that does not shrink in water and leads to immobilized enzyme that shows high activity in the hydrolysis of both low and high molecular weight substrates such as L-benzoyl arginine ethyl ester and casein. The catalytic activity of the monolithic reactor is maintained even at a flow velocity of 180 cm/min, which substantially exceeds those reported in the literature for packed bed reactors. | lld:pubmed |
| pubmed-article:10099510 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10099510 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10099510 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10099510 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10099510 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10099510 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10099510 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10099510 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10099510 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:10099510 | pubmed:issn | 0006-3592 | lld:pubmed |
| pubmed-article:10099510 | pubmed:author | pubmed-author:SvecFF | lld:pubmed |
| pubmed-article:10099510 | pubmed:author | pubmed-author:XieSS | lld:pubmed |
| pubmed-article:10099510 | pubmed:author | pubmed-author:FréchetJ MJM | lld:pubmed |
| pubmed-article:10099510 | pubmed:copyrightInfo | Copyright 1999 John Wiley & Sons, Inc. | lld:pubmed |
| pubmed-article:10099510 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10099510 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:10099510 | pubmed:volume | 62 | lld:pubmed |
| pubmed-article:10099510 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10099510 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10099510 | pubmed:pagination | 30-5 | lld:pubmed |
| pubmed-article:10099510 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:10099510 | pubmed:meshHeading | pubmed-meshheading:10099510... | lld:pubmed |
| pubmed-article:10099510 | pubmed:meshHeading | pubmed-meshheading:10099510... | lld:pubmed |
| pubmed-article:10099510 | pubmed:meshHeading | pubmed-meshheading:10099510... | lld:pubmed |
| pubmed-article:10099510 | pubmed:meshHeading | pubmed-meshheading:10099510... | lld:pubmed |
| pubmed-article:10099510 | pubmed:meshHeading | pubmed-meshheading:10099510... | lld:pubmed |
| pubmed-article:10099510 | pubmed:meshHeading | pubmed-meshheading:10099510... | lld:pubmed |
| pubmed-article:10099510 | pubmed:meshHeading | pubmed-meshheading:10099510... | lld:pubmed |
| pubmed-article:10099510 | pubmed:meshHeading | pubmed-meshheading:10099510... | lld:pubmed |
| pubmed-article:10099510 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10099510 | pubmed:articleTitle | Design of reactive porous polymer supports for high throughput bioreactors: poly(2-vinyl-4,4-dimethylazlactone-co-acrylamide- co-ethylene dimethacrylate) monoliths. | lld:pubmed |
| pubmed-article:10099510 | pubmed:affiliation | Department of Chemistry, University of California, 718 Latimer Hall, Berkeley, California 94720-1460, USA. | lld:pubmed |
| pubmed-article:10099510 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10099510 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:10099510 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10099510 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10099510 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10099510 | lld:pubmed |
