Source:http://linkedlifedata.com/resource/pubmed/id/10099510
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1999-5-7
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pubmed:abstractText |
Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4, 4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlactone functional groups. Optimization of hydrophilic/hydrophobic properties of the monolith affords a support that does not shrink in water and leads to immobilized enzyme that shows high activity in the hydrolysis of both low and high molecular weight substrates such as L-benzoyl arginine ethyl ester and casein. The catalytic activity of the monolithic reactor is maintained even at a flow velocity of 180 cm/min, which substantially exceeds those reported in the literature for packed bed reactors.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-3592
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 1999 John Wiley & Sons, Inc.
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30-5
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10099510-Bioreactors,
pubmed-meshheading:10099510-Biotechnology,
pubmed-meshheading:10099510-Drug Design,
pubmed-meshheading:10099510-Enzymes, Immobilized,
pubmed-meshheading:10099510-Kinetics,
pubmed-meshheading:10099510-Polymers,
pubmed-meshheading:10099510-Substrate Specificity,
pubmed-meshheading:10099510-Trypsin
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pubmed:year |
1999
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pubmed:articleTitle |
Design of reactive porous polymer supports for high throughput bioreactors: poly(2-vinyl-4,4-dimethylazlactone-co-acrylamide- co-ethylene dimethacrylate) monoliths.
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pubmed:affiliation |
Department of Chemistry, University of California, 718 Latimer Hall, Berkeley, California 94720-1460, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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