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pubmed-article:1009933pubmed:abstractTextMammalian RNA polymerase B is able to initiate at single-strand breaks in the DNA template. A 3'-OH end at a nick is required for initiation whereas the 5'-end may be either -- OH or phosphate. The 3'-OH group does not function as a primer. An appreciable part of the newly synthesized RNA started at a nick remains associated with the DNA in hydrid form. Initiation of exogeneous RNA polymerase B on chromatin exhibits similar requirements.lld:pubmed
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pubmed-article:1009933pubmed:articleTitleOn the initiation of mammalian RNA polymerase at single-strand breaks in DNA.lld:pubmed
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