Source:http://linkedlifedata.com/resource/pubmed/id/10096786
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1999-5-17
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pubmed:abstractText |
The intensity and mechanisms of cell-mediated rejection of pig islet cells were studied in 49 Type I diabetic and 34 healthy subjects. Human peripheral mononuclear cells proliferated strongly in response to pig islet cells (p<0.001), though with notable interindividual variations (stimulation index 2 to 215). The variance of stimulation index was higher in diabetic than healthy subjects (p<0.0001). The response to islet cells was stronger (p<0.01) than that to pig splenocytes. Proliferation in response to islet cells was strongly decreased (p<0.01) when CD4+ T cells were blocked with monoclonal antibodies, whereas the blocking of CD8+ cells or NK cells gave less pronounced effects. The response to islet cells was decreased (p<0.01), but not abolished, after antigen-presenting cells were removed. Purified CD4+ cells alone did not proliferate in response to islet cells but recovered their proliferative ability when mixed with antigen-presenting cells, whereas CD8+ cells alone proliferated in the presence of interleukin-2 in response to islet cells. Proliferation was blocked (p<0.01) by anti-DR monoclonal antibodies. During proliferation in response to islet cells, interleukin-10 increased 43-fold (p<0.01) but interferon-gamma increased only slightly. No statistical differences were detected between diabetic and control subjects with respect to lymphocyte subsets and the recognition mechanisms or to interferon-gamma/interleukin-10 production in response to islet cells. These results provide the first detailed information on human cell-mediated xenoreaction to pig islet cells. This situation involves a dominant CD4 class II-restricted Th2 response, with an indirect recognition pathway, as well as a CD8 T-cell response resulting from direct recognition. This strong reaction constitutes a serious obstacle which may vary in degree among subjects.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0012-186X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
330-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10096786-Adult,
pubmed-meshheading:10096786-Animals,
pubmed-meshheading:10096786-Antibodies, Monoclonal,
pubmed-meshheading:10096786-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10096786-CD8-Positive T-Lymphocytes,
pubmed-meshheading:10096786-Cells, Cultured,
pubmed-meshheading:10096786-Diabetes Mellitus, Type 1,
pubmed-meshheading:10096786-Female,
pubmed-meshheading:10096786-Humans,
pubmed-meshheading:10096786-Islets of Langerhans,
pubmed-meshheading:10096786-Killer Cells, Natural,
pubmed-meshheading:10096786-Lymphocyte Activation,
pubmed-meshheading:10096786-Male,
pubmed-meshheading:10096786-Reference Values,
pubmed-meshheading:10096786-Spleen,
pubmed-meshheading:10096786-Swine,
pubmed-meshheading:10096786-Transplantation, Heterologous
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pubmed:year |
1999
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pubmed:articleTitle |
Intensity and mechanisms of in vitro xenorecognition of adult pig pancreatic islet cells by CD4+ and CD8+ lymphocytes from type I diabetic or healthy subjects.
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pubmed:affiliation |
Cellular and Molecular Immuno-Endocrinology, University/INRA/ENVN, Nantes, France.
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pubmed:publicationType |
Journal Article,
In Vitro
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