Linked Life Data

10091941

Source:http://linkedlifedata.com/resource/pubmed/id/10091941

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-4-15
pubmed:abstractText
Human colonic carcinoma cell lines, KM12C, KM12SM and KM12L4, were previously established and their in vivo metastatic potentials have been well evaluated. The highly metastatic cell lines KM12SM and KM12L4 were derived from the parental low metastatic cell line KM12C in vivo. To evaluate the metastatic behavior of these cell lines in vitro, we examined colony formation on monolayers of the pulmonary arterial endothelial (CPAE) cells. On day 4, the highly metastatic cell lines showed an approximately 2-fold increase in number of colonies on CPAE cell monolayers relative to the parental KM12C cell line. To investigate what evidence is correlated with their metastatic and invasive abilities, Northern blot analysis and flow cytometry were performed in all cell lines. According to the results of Northern blot analysis, the levels of matrix metalloproteinase (MMP)-2 and c-met mRNA expression were increased in highly metastatic cell lines as compared with the parental cell line. We also examined the cell-surface expression of several adhesion molecules by flow cytometry. The levels of expression of sialyl Lewisa antigen (sLe(a)) in KM12SM and KM12L4 were twice higher than that in KM12C. However, the levels of expression of E-cadherin in KM12SM and KM12L4 were decreased to half that in KM12C. The alterative expression of the collagenase and adhesion molecules might contribute to their metastatic/invasive abilities of these cell lines both in vivo and in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0262-0898
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
461-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10091941-Antigens, CD, pubmed-meshheading:10091941-Antigens, CD44, pubmed-meshheading:10091941-Antigens, Neoplasm, pubmed-meshheading:10091941-Blotting, Northern, pubmed-meshheading:10091941-Cadherins, pubmed-meshheading:10091941-Cell Adhesion Molecules, pubmed-meshheading:10091941-Cell Communication, pubmed-meshheading:10091941-Cell Line, pubmed-meshheading:10091941-Collagenases, pubmed-meshheading:10091941-Colonic Neoplasms, pubmed-meshheading:10091941-Endothelium, Vascular, pubmed-meshheading:10091941-Flow Cytometry, pubmed-meshheading:10091941-Humans, pubmed-meshheading:10091941-Integrin alpha3, pubmed-meshheading:10091941-Integrins, pubmed-meshheading:10091941-Matrix Metalloproteinase 1, pubmed-meshheading:10091941-Neoplasm Metastasis, pubmed-meshheading:10091941-Tissue Inhibitor of Metalloproteinase-2, pubmed-meshheading:10091941-Tumor Cells, Cultured
pubmed:year
1998
pubmed:articleTitle
Alterative expression of the collagenase and adhesion molecules in the highly metastatic clones of human colonic cancer cell lines.
pubmed:affiliation
Department of Surgery 1, University of Occupational and Environmental Health, Kita-kyushu, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't