|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1999-4-15
|
|
pubmed:abstractText |
The protein kinase Chk1 is required for cell cycle arrest in response to DNA damage. We have found that the 14-3-3 proteins Rad24 and Rad25 physically interact with Chk1 in fission yeast. Association of Chk1 with 14-3-3 proteins is stimulated in response to DNA damage. DNA damage results in phosphorylation of Chk1 and the 14-3-3 proteins bind preferentially to the phosphorylated form. Genetic analysis has independently implicated both Rad24 and Rad25 in the DNA-damage checkpoint pathway. We suggest that DNA damage-dependent association of phosphorylated Chk1 with 14-3-3 proteins mediates an important step along the DNA-damage checkpoint pathway, perhaps by directing Chk1 to a particular substrate or to a particular location within the cell. An additional role for 14-3-3 proteins in the DNA-damage checkpoint has been suggested based on the observation that human Chk1 can phosphorylate Cdc25C in vitro creating a 14-3-3 binding site. Our results suggest that in fission yeast the interaction between the 14-3-3 proteins and Cdc25 does not require Chk1 function and is unaffected by DNA damage, in sharp contrast to the interaction between the 14-3-3 proteins and Chk1.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-1427071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-15157431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-1563349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-1563350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-2005825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-2682257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-2683079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-3047011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-3291120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-7603573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-7603574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-7644510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-7760835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-7792600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-7926756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-8019001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-8036497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-8422996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-8497322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-8514150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-8553071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-8601312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-8631758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-8635463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-8663231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-8939848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-9001228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-9034337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-9042863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-9251017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-9278510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-9278511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-9278512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-9304216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-9450960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-9543386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-9744884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10090724-9774107
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Checkpoint kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Schizosaccharomyces pombe Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/rad24 protein, S pombe,
http://linkedlifedata.com/resource/pubmed/chemical/rad25 protein, S pombe
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0890-9369
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
13
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
675-85
|
|
pubmed:dateRevised |
2011-11-2
|
|
pubmed:meshHeading |
pubmed-meshheading:10090724-Base Sequence,
pubmed-meshheading:10090724-Cell Cycle,
pubmed-meshheading:10090724-Cell Cycle Proteins,
pubmed-meshheading:10090724-DNA Damage,
pubmed-meshheading:10090724-DNA Helicases,
pubmed-meshheading:10090724-DNA Primers,
pubmed-meshheading:10090724-Fungal Proteins,
pubmed-meshheading:10090724-Humans,
pubmed-meshheading:10090724-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:10090724-Phosphorylation,
pubmed-meshheading:10090724-Protein Binding,
pubmed-meshheading:10090724-Protein Kinases,
pubmed-meshheading:10090724-Recombinant Proteins,
pubmed-meshheading:10090724-Schizosaccharomyces,
pubmed-meshheading:10090724-Schizosaccharomyces pombe Proteins
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Association of Chk1 with 14-3-3 proteins is stimulated by DNA damage.
|
|
pubmed:affiliation |
Department of Pharmacology, University of Medicine and Dentistry of New Jersey (UMDNJ)-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
