Linked Life Data

10090338

Source:http://linkedlifedata.com/resource/pubmed/id/10090338

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 1
pubmed:dateCreated
1999-5-21
pubmed:abstractText
The main aims of this work were to examine in women: the relationship between the freely exchangeable Ca2+ (FECa2+) in the dense tubules and the activity of the sarco(endo)plasmic reticulum (SER) Ca2+-ATPase (SERCA) in platelets, and the relationship of these parameters with blood pressure and serum lipoproteins. Platelets from 14 white and 13 black women in good health were studied. The FECa2+ was measured as the ionomycin-evoked Ca2+ release (in the presence of thapsigargin) in Ca2+-free medium. SERCA activity was measured as the thapsigargin sensitive, Ca2+ dependent and ouabain resistant, ATP hydrolyses in platelet membranes. Relative expressions of SERCA 2 and 3 isoforms and Ras-related protein (Rap) 1 in platelet membranes were determined by Western immunoblots. Highly significant correlations were observed for FECa2+ in the dense tubules with: 1) the maximal reaction velocity (Vmax) of the SERCA (r = 0.592, P = .0014), and 2) Rapl (r = 0.551, P = .0035). In addition, negative correlations were observed between FECa2+ in the dense tubules and age. No correlations were observed for these variables with blood pressure or serum lipoproteins. We conclude the FECa2+ and the Vmax of the SERCA are reliable indicators of Ca2+ load in platelets from women. However, in women, unlike previous observations in men, these platelet parameters are not correlated with blood pressure and serum lipoproteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0895-7061
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
120-7
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:10090338-Adult, pubmed-meshheading:10090338-African Continental Ancestry Group, pubmed-meshheading:10090338-Blood Platelets, pubmed-meshheading:10090338-Blood Pressure, pubmed-meshheading:10090338-Blotting, Western, pubmed-meshheading:10090338-Calcium, pubmed-meshheading:10090338-Calcium-Transporting ATPases, pubmed-meshheading:10090338-Cell Membrane, pubmed-meshheading:10090338-Cytosol, pubmed-meshheading:10090338-Enzyme Inhibitors, pubmed-meshheading:10090338-European Continental Ancestry Group, pubmed-meshheading:10090338-Female, pubmed-meshheading:10090338-GTP-Binding Proteins, pubmed-meshheading:10090338-Humans, pubmed-meshheading:10090338-Hypertension, pubmed-meshheading:10090338-Ionomycin, pubmed-meshheading:10090338-Ionophores, pubmed-meshheading:10090338-Lipoproteins, pubmed-meshheading:10090338-Sarcoplasmic Reticulum, pubmed-meshheading:10090338-Transcription Factors, pubmed-meshheading:10090338-rap GTP-Binding Proteins
pubmed:year
1999
pubmed:articleTitle
The relationship between Ca2+-ATPase and freely exchangeable Ca2+ in the dense tubules: a study in platelets from women.
pubmed:affiliation
Hypertension Research Center, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 071032-2714, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't