Linked Life Data

10089156

Source:http://linkedlifedata.com/resource/pubmed/id/10089156

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-5-6
pubmed:abstractText
The pathogenic Yersiniae produce a range of virulence proteins, encoded by a 70 kb plasmid, which are essential for infection, and also form part of a contact-dependent virulence mechanism. One of these proteins, V antigen, has been shown to confer a high level of protection against parenteral infection with Y. pestis in murine models, and is considered to be a protective antigen. In this study, the protective efficacy of V antigen has been compared in the same model with that of other proteins (YopE, YopK and YopN), which are part of the contact-dependent virulence mechanism. Mice immunised with two intraperitoneal doses of V antigen or each of the Yops, administered with either Alhydrogel or interleukin-12, produced high antigen-specific serum IgG titres. As shown in previous studies, V+Alhydrogel was fully protective, and 5/5 mice survived a subcutaneous challenge with 90 or 9x10(3) LD50's of Y. pestis GB. In addition, these preliminary studies also showed that V+IL-12 was partially protective: 4/5 or 3/5 mice survived a challenge with 90 or 9x10(3) LD50's, respectively. In contrast, none of the mice immunised with the Yops survived the challenges, and there was no significant delay in the mean time to death compared to mice receiving a control protein. These results show that using two different vaccine regimens, Yops E, K and N, failed to elicit protective immune responses in a murine model of plague, whereas under the same conditions, V antigen was fully or partially protective.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0882-4010
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
159-69
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10089156-Animals, pubmed-meshheading:10089156-Antibodies, Bacterial, pubmed-meshheading:10089156-Antibody Specificity, pubmed-meshheading:10089156-Antigens, Bacterial, pubmed-meshheading:10089156-Bacterial Outer Membrane Proteins, pubmed-meshheading:10089156-Bacterial Proteins, pubmed-meshheading:10089156-Disease Models, Animal, pubmed-meshheading:10089156-Female, pubmed-meshheading:10089156-Immunoglobulin G, pubmed-meshheading:10089156-Membrane Proteins, pubmed-meshheading:10089156-Mice, pubmed-meshheading:10089156-Mice, Inbred BALB C, pubmed-meshheading:10089156-Plague, pubmed-meshheading:10089156-Pore Forming Cytotoxic Proteins, pubmed-meshheading:10089156-Specific Pathogen-Free Organisms, pubmed-meshheading:10089156-Yersinia, pubmed-meshheading:10089156-Yersinia pestis
pubmed:year
1999
pubmed:articleTitle
Yersinia outer proteins (YOPS) E, K and N are antigenic but non-protective compared to V antigen, in a murine model of bubonic plague.
pubmed:affiliation
Biomedical Sciences Department, Defence Evaluation and Research Agency, Porton Down, Salisbury, Wiltshire, SP4 0JQ, U.K.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't