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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-5-21
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pubmed:abstractText |
The compound 4-(fluorosulfonyl)benzoic acid (4-FSB) functions as an affinity label of the dimeric pig lung pi class glutathione S-transferase yielding a completely inactive enzyme. Protection against inactivation is provided by glutathione-based ligands, suggesting that the reaction target is near or part of the glutathione binding site. Radioactive 4-FSB is incorporated to the extent of 1 mol per mole of enzyme subunit. Peptide mapping revealed that 4-FSB reacts with two tyrosine residues in the ratio 69% Tyr7 and 31% Tyr106. The ratio is not changed by the addition of ligands. The results suggest that only one of the tyrosine residues can be labeled in the active site of a given subunit; i.e., reactions with Tyr7 and Tyr106 are mutually exclusive. We propose that the difference in labeling of these tyrosine residues is related to their pKa values, with Tyr7 exhibiting the lower pKa. The modified enzyme no longer binds to a S-hexylglutathione-agarose affinity column, even when only one of the active sites contains 4-FSB; these results may reflect interaction between the subunits. We conclude that Tyr7 and Tyr106 of the pig lung class pi glutathione S-transferase are important for function and are located at or close to the substrate binding site of the enzyme.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0003-9861
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
364
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
107-14
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10087171-Affinity Labels,
pubmed-meshheading:10087171-Amino Acid Sequence,
pubmed-meshheading:10087171-Animals,
pubmed-meshheading:10087171-Benzoic Acids,
pubmed-meshheading:10087171-Binding Sites,
pubmed-meshheading:10087171-Chromatography, Affinity,
pubmed-meshheading:10087171-Chromatography, High Pressure Liquid,
pubmed-meshheading:10087171-Dose-Response Relationship, Drug,
pubmed-meshheading:10087171-Enzyme Activation,
pubmed-meshheading:10087171-Glutathione S-Transferase pi,
pubmed-meshheading:10087171-Glutathione Transferase,
pubmed-meshheading:10087171-Isoenzymes,
pubmed-meshheading:10087171-Ligands,
pubmed-meshheading:10087171-Lung,
pubmed-meshheading:10087171-Molecular Sequence Data,
pubmed-meshheading:10087171-Peptide Fragments,
pubmed-meshheading:10087171-Sequence Analysis,
pubmed-meshheading:10087171-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:10087171-Swine,
pubmed-meshheading:10087171-Time Factors,
pubmed-meshheading:10087171-Tyrosine
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pubmed:year |
1999
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pubmed:articleTitle |
Affinity labeling of pig lung glutathione S-transferase pi by 4-(fluorosulfonyl)benzoic acid.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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