Source:http://linkedlifedata.com/resource/pubmed/id/10086790
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1999-4-15
|
pubmed:abstractText |
Patients with aplastic anaemia (AA) frequently develop paroxysmal nocturnal haemoglobinuria (PNH) as a late complication. We investigated the frequency of the development of PNH features including a glycosyl phosphatidylinositol (GPI) anchoring defect in 73 Japanese patients with AA. A deficient expression of CD59 was found on erythrocytes and/or granulocytes in 21/73 (28.8%) of the patients. A Ham/sugar water test was positive in 13/21 patients. We also examined mutations of the PIG-A gene in 11 patients with CD59 deficiency. A heteroduplex analysis detected PIG-A gene abnormality in 10/11 patients tested. Nucleotide sequencing was performed in six patients and identified eight mutations including three mutations in one patient. The mutations of the PIG-A gene were all different and included two single-base insertions, one single-base deletion, two two-base deletions, and one each of eight-base insertion and nine- and ten-base deletions. All mutations but one caused frameshifts. Our findings indicate that a high proportion of Japanese patients with severe AA have a GPI-anchoring defect and that the PIG-A gene is mutated in the AA patients who had a GPI deficiency. We found no significant difference in the pattern of the PIG-A gene mutation between the AA patients with a GPI deficiency and those with de novo PNH.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD59,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosylphosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/phosphatidylinositol glycan-class...
|
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0007-1048
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
104
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
523-9
|
pubmed:dateRevised |
2004-11-17
|
pubmed:meshHeading |
pubmed-meshheading:10086790-Adolescent,
pubmed-meshheading:10086790-Adult,
pubmed-meshheading:10086790-Aged,
pubmed-meshheading:10086790-Anemia, Aplastic,
pubmed-meshheading:10086790-Antigens, CD59,
pubmed-meshheading:10086790-Erythrocytes,
pubmed-meshheading:10086790-Female,
pubmed-meshheading:10086790-Glycosylphosphatidylinositols,
pubmed-meshheading:10086790-Granulocytes,
pubmed-meshheading:10086790-Hemoglobinuria, Paroxysmal,
pubmed-meshheading:10086790-Humans,
pubmed-meshheading:10086790-Japan,
pubmed-meshheading:10086790-Male,
pubmed-meshheading:10086790-Membrane Proteins,
pubmed-meshheading:10086790-Middle Aged,
pubmed-meshheading:10086790-Mutation
|
pubmed:year |
1999
|
pubmed:articleTitle |
CD59-deficient blood cells and PIG-A gene abnormalities in Japanese patients with aplastic anaemia.
|
pubmed:affiliation |
Haematology and Oncology, Osaka University Medical School, Suita, Japan.
|
pubmed:publicationType |
Journal Article
|