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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1999-4-15
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pubmed:abstractText |
11 beta-Hydroxysteroid dehydrogenase (HSD) activity was measured in freshly frozen granulosa cells isolated from follicles of twenty-one infertility patients undergoing in vitro fertilization-embryo transfer (IVF-ET). A total of 213 follicles were analyzed for 11 beta-HSD activity. Both nicotinamide-adenine dinucleotide (NAD) and nicotinamide-adenine dinucleotide phosphate (NADP) dependent 11 beta-dehydrogenase activities were measured in granulosa cells. The activity in reductive direction (11-oxoreductase activity) was not measurable either with NADH or NADPH as cofactors. NAD- and NADP-dependent dehydrogenase activities are in comparable levels at 100 nmol/l and 1 mumol/l corticosteroid concentrations. For comparing enzyme activities of individual follicles, significant enzyme activity was considered to be a level of > 2 nmol/l/min/mg. 41.3% of the follicles demonstrated enzyme activity, 58.7% did not. The mean 11 beta-dehydrogenase (11 beta-DH) activity was calculated for each patient. Those patients with levels > 2 nmol/l/min/mg were considered enzyme positive; those with mean levels < 2 nmol/l/min/mg were considered negative. No significant association was noted between follicle size, oocyte maturity or fertilization rates and 11 beta-DH activity. This study noted the presence of 11 beta-DH activity in granulosa cells, however, no association with oocyte maturity and fertilization was found.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1354-4195
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
183-9
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:10086068-11-beta-Hydroxysteroid Dehydrogenases,
pubmed-meshheading:10086068-Adult,
pubmed-meshheading:10086068-Chromatography, Thin Layer,
pubmed-meshheading:10086068-Corticosterone,
pubmed-meshheading:10086068-Embryo Transfer,
pubmed-meshheading:10086068-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10086068-Estradiol,
pubmed-meshheading:10086068-Female,
pubmed-meshheading:10086068-Fertilization in Vitro,
pubmed-meshheading:10086068-Follicle Stimulating Hormone,
pubmed-meshheading:10086068-Follicular Fluid,
pubmed-meshheading:10086068-Granulosa Cells,
pubmed-meshheading:10086068-Humans,
pubmed-meshheading:10086068-Hydrocortisone,
pubmed-meshheading:10086068-Hydroxysteroid Dehydrogenases,
pubmed-meshheading:10086068-Luteinizing Hormone,
pubmed-meshheading:10086068-NAD,
pubmed-meshheading:10086068-NADP,
pubmed-meshheading:10086068-Ovarian Follicle,
pubmed-meshheading:10086068-Pregnancy,
pubmed-meshheading:10086068-Pregnancy Outcome,
pubmed-meshheading:10086068-Progesterone,
pubmed-meshheading:10086068-Scintillation Counting
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pubmed:year |
1997
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pubmed:articleTitle |
11 beta-Hydroxysteroid dehydrogenase enzyme activity in granulosa cells derived from ovarian follicles of in vitro fertilization patients.
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pubmed:affiliation |
Princeton Center for Infertility and Reproductive Medicine, Lawrenceville, NJ 08648, USA.
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pubmed:publicationType |
Journal Article
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