Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-4-16
pubmed:abstractText
Thirty-six cows (21 Simmental, 5 Holstein-Frisian, 5 Brown Swiss and 5 Charolais) with high genetic superiority were punctured by ultrasound-guided follicle aspiration within the last 18 months for 503 times under equal conditions. Follicle aspiration was done twice per week. Most of the donor cows suffered from several disturbances of fertility. On average, 5 oocytes per session were collected. After in vitro maturation (IVM), fertilisation (IVF) and culture (IVC), 0.8 embryos per puncture session were transferred. After evaluation of the embryos by morphological criteria, these embryos were transferred to heifers that were oestrus synchronised (2.0 ml Estrumate i.m.) seven days after onset of oestrus. On day 21 after onset of oestrus the progesterone level in plasma was determined by radioimmunoassay. The pregnancy control was performed by ultrasound on day 35. After transfer of 397 embryos (to synchronised heifers), 125 pregnancies were established. Comparison of the different breeds and donor cows revealed significant differences in the number of oocytes recovered, embryos produced and pregnancies established. Transfer of embryos with insufficient morphological quality resulted in increased rates of embryonic loss between day 21 and day 35.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0514-7158
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Pregnancy rate after ultrasound-guided follicle aspiration in nonlactating cows from different breeds.
pubmed:affiliation
Bavarian Research Centre of Biology of Reproduction, Oberschissheim, Germany. w.schernthaner@gen.vetmed.uni-muenchen.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't