Linked Life Data

10085265

Source:http://linkedlifedata.com/resource/pubmed/id/10085265

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 8
pubmed:dateCreated
1999-6-21
pubmed:abstractText
The nourishment received by female honeybee larvae determines their differentiation into queens or workers. In this study, we report the first molecular analysis of differences that occur between queens and workers during the caste-determination process. RNA-differential display experiments identified a clone that encodes for a gene that is homologous to the nuclear-encoded mitochondrial translation initiation factor (AmIF-2mt). Semi-quantitative analysis by reverse transcriptase/polymerase chain reaction (RT-PCR) throughout honeybee development detected a higher level of expression of this gene in queen larvae than in worker larvae. Analysis of two other genes encoding mitochondrial proteins, cytochrome oxidase subunit 1 (COX-1; mitochondrial-encoded) and cytochrome c (cyt c; nuclear-encoded) also showed differential expression of these two genes between queens and workers. In particular, the cyt c transcript is more abundant in queen larvae and throughout the metamorphosis of the queen. These results indicate that the higher respiratory rate previously documented in queen larvae is accomplished through a higher level of expression of both nuclear- and mitochondrial-encoded genes for mitochondrial proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-0949
pubmed:author
pubmed:issnType
Print
pubmed:volume
202
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
929-38
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10085265-Amino Acid Sequence, pubmed-meshheading:10085265-Animals, pubmed-meshheading:10085265-Base Sequence, pubmed-meshheading:10085265-Bees, pubmed-meshheading:10085265-Cytochrome c Group, pubmed-meshheading:10085265-DNA, Complementary, pubmed-meshheading:10085265-DNA, Mitochondrial, pubmed-meshheading:10085265-DNA Primers, pubmed-meshheading:10085265-Electron Transport Complex IV, pubmed-meshheading:10085265-Female, pubmed-meshheading:10085265-Gene Expression Regulation, Developmental, pubmed-meshheading:10085265-Genes, Insect, pubmed-meshheading:10085265-Insect Proteins, pubmed-meshheading:10085265-Molecular Sequence Data, pubmed-meshheading:10085265-Peptide Initiation Factors, pubmed-meshheading:10085265-RNA, Messenger, pubmed-meshheading:10085265-Sequence Homology, Amino Acid
pubmed:year
1999
pubmed:articleTitle
Differential expression of mitochondrial genes between queens and workers during caste determination in the honeybee Apis mellifera.
pubmed:affiliation
Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, APDO-Postal 510-3, Cuernavaca Morelos 62250, México.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't