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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1999-4-29
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pubmed:abstractText |
Cripto-1 (CR-1) is a recently discovered protein of the epidermal growth factor family that fails to directly bind to any of the four known erb B type 1 receptor tyrosine kinases. The present study demonstrates that CR-1 indirectly induces tyrosine phosphorylation of erb B-4 but not of the epidermal growth factor-related receptors erb B-2 and erb B-3 in different mouse and human mammary epithelial cell lines. In addition, down-regulation of erb B-4 in NMuMG mouse mammary epithelial cells and in T47D human breast cancer cells, using an anti-erb B-4 blocking antibody or a hammerhead ribozyme vector targeted to erb B-4 mRNA, impairs the ability of CR-1 to fully activate mitogen-activated protein kinase. Finally, chemical cross-linking of 125I-CR-1 to mouse and human mammary epithelial cell membranes results in the labeling of two specific bands with a molecular weight of 130 and 60 kDa, suggesting that the CR-1 receptor represents a novel receptor structurally unrelated to any of the known type I receptor tyrosine kinases. In conclusion, these data demonstrate that CR-1, upon binding to an unknown receptor, can enhance the tyrosine kinase activity of erb B-4 and that a functional erb B-4 receptor is required for CR-1-induced MAPK activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GPI-Linked Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neuregulin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Catalytic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Succinimides,
http://linkedlifedata.com/resource/pubmed/chemical/TDGF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tdgf1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/bis(sulfosuccinimidyl)suberate,
http://linkedlifedata.com/resource/pubmed/chemical/heregulin beta1,
http://linkedlifedata.com/resource/pubmed/chemical/receptor tyrosine-protein kinase...
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:BiancoCC,
pubmed-author:De SantisMM,
pubmed-author:EbertA DAD,
pubmed-author:KannanSS,
pubmed-author:KimNN,
pubmed-author:LippmanM EME,
pubmed-author:Martinez-LacaciII,
pubmed-author:SalomonD SDS,
pubmed-author:SenoMM,
pubmed-author:TangC KCK,
pubmed-author:Wallace-JonesBB,
pubmed-author:WechselbergerCC
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8624-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10085099-Animals,
pubmed-meshheading:10085099-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:10085099-Carrier Proteins,
pubmed-meshheading:10085099-Cell Line,
pubmed-meshheading:10085099-Cross-Linking Reagents,
pubmed-meshheading:10085099-Enzyme Activation,
pubmed-meshheading:10085099-Epidermal Growth Factor,
pubmed-meshheading:10085099-GPI-Linked Proteins,
pubmed-meshheading:10085099-Glycoproteins,
pubmed-meshheading:10085099-Humans,
pubmed-meshheading:10085099-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:10085099-Membrane Glycoproteins,
pubmed-meshheading:10085099-Mice,
pubmed-meshheading:10085099-Neoplasm Proteins,
pubmed-meshheading:10085099-Neuregulin-1,
pubmed-meshheading:10085099-Phosphorylation,
pubmed-meshheading:10085099-Protein-Tyrosine Kinases,
pubmed-meshheading:10085099-RNA, Catalytic,
pubmed-meshheading:10085099-Receptor, Epidermal Growth Factor,
pubmed-meshheading:10085099-Receptors, Cell Surface,
pubmed-meshheading:10085099-Succinimides
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pubmed:year |
1999
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pubmed:articleTitle |
Cripto-1 indirectly stimulates the tyrosine phosphorylation of erb B-4 through a novel receptor.
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pubmed:affiliation |
Tumor Growth Factor Section, Laboratory of Tumor Immunology and Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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