Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-4-26
pubmed:databankReference
pubmed:abstractText
To study the role of the capsule of Streptococcus suis serotype 2 in virulence, we generated two isogenic mutants disturbed in capsule production. For that purpose, we first cloned and characterized a major part of the capsular polysaccharide biosynthesis (cps) locus of S. suis serotype 2. Based on the established sequence, 14 open reading frames (ORFs), designated Orf2Z, Orf2Y, Orf2X, and Cps2A to Cps2K, were identified. Twelve ORFs belonged to a single transcriptional unit. The gene products of 11 of these ORFs showed similarity to proteins involved in polysaccharide biosynthesis of other gram-positive microorganisms. Nonencapsulated isogenic mutants were generated in the cps2B and cps2EF genes by insertional mutagenesis. In contrast to the wild-type S. suis serotype 2 strain, the nonencapsulated strains were highly sensitive to ingestion by porcine alveolar lung macrophages in vitro. More importantly, the nonencapsulated mutant strains were completely avirulent in young germfree pigs after intranasal inoculation. These observations indicate that the capsule of S. suis serotype 2 plays an essential role in the pathogenesis of S. suis serotype 2 infections.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-1730489, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-1774266, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-1879937, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-1937823, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-1971617, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-1977767, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-2526407, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-2660110, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-2774320, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-2999794, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-3323801, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-3353625, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-3617416, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-363973, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-4071952, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-6099327, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-6839111, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-7506702, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-7578463, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-7894710, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-7894711, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-7934910, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-7960118, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-8051253, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-8125242, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-8168935, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-8566758, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-8626297, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-8682774, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-8828129, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-8905082, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-8926123, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-8969502, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-9159524, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-9235953, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-9383201, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-9466257, http://linkedlifedata.com/resource/pubmed/commentcorrection/10085014-9493370
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1750-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Identification and characterization of the cps locus of Streptococcus suis serotype 2: the capsule protects against phagocytosis and is an important virulence factor.
pubmed:affiliation
Department of Bacteriology, Pathobiology and Epidemiology, DLO-Institute for Animal Science and Health, 8200 AB Lelystad, The Netherlands. h.e.smith@id.dlo.nl
pubmed:publicationType
Journal Article