Source:http://linkedlifedata.com/resource/pubmed/id/10082809
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
1999-5-6
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| pubmed:abstractText |
We have identified by Scatchard analysis both high (124 pM, 14.4 x106 sites/micrograms protein, 7600 sites/cell) and low (1.6 nM, 7.7x106 sites/micrograms protein, 4100 sites/cell) affinity receptors for [125I]-rat ciliary neurotrophic factor (rCNTF) on astrocytes. Ligand competition studies showed that the binding of [125I]-rCNTF was effectively competed by rCNTF and human CNTF, but not by hLIF, mIL-6 or mIL-1B. Three proteins specifically crossed-linked to [125I]-rCNTF, with the molecular weights of 190, 100, and 43 kDa, were immunoprecipitated by anti-rCNTF antibodies. Anti-LIFR or anti-gp130 antibodies immunoprecipitated the 100 and the 190 kDa proteins. CNTF induced the tyrosine phosphorylation of LIFR and gp130, as well as of proteins with the molecular weights of 88/91 and 42 kDa. The phosphorylation of the 88/91 kDa protein(s) was inhibited by pretreating the cells with staurosporine, 12-myristate 13-acetate phorbol (PMA), W7, chlorpromazine, or the intracellular Ca+2 chelator BAPTA/AM. In contrast, CNTF and PMA acted synergistically to induce the phosphorylation of two proteins with the molecular weights of 42 and 44 kDa. At later time points following CNTF treatment, c-fos messenger RNA and protein levels were increased. Collectively, these data indicate that hippocampal astrocytes express high-affinity, biologically functional receptor complexes for CNTF.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ciliary Neurotrophic Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0006-8993
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1999 Elsevier Science B.V.
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| pubmed:issnType |
Print
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| pubmed:day |
13
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| pubmed:volume |
818
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
236-51
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:10082809-Animals,
pubmed-meshheading:10082809-Astrocytes,
pubmed-meshheading:10082809-Binding, Competitive,
pubmed-meshheading:10082809-Cells, Cultured,
pubmed-meshheading:10082809-Ciliary Neurotrophic Factor,
pubmed-meshheading:10082809-Endocytosis,
pubmed-meshheading:10082809-Enzyme Inhibitors,
pubmed-meshheading:10082809-Hippocampus,
pubmed-meshheading:10082809-Humans,
pubmed-meshheading:10082809-Nerve Growth Factors,
pubmed-meshheading:10082809-Nerve Tissue Proteins,
pubmed-meshheading:10082809-Phorbol Esters,
pubmed-meshheading:10082809-Phosphatidylinositols,
pubmed-meshheading:10082809-Phosphorylation,
pubmed-meshheading:10082809-Protein-Tyrosine Kinases,
pubmed-meshheading:10082809-Radioligand Assay,
pubmed-meshheading:10082809-Rats,
pubmed-meshheading:10082809-Rats, Sprague-Dawley,
pubmed-meshheading:10082809-Type C Phospholipases
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| pubmed:year |
1999
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| pubmed:articleTitle |
Characterization of receptors for ciliary neurotrophic factor on rat hippocampal astrocytes.
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| pubmed:affiliation |
Regeneron Pharmaceuticals, 777 Old Saw Mill River Road, Tarrytown, NY., USA. ralph_alderson@hgsi.com
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| pubmed:publicationType |
Journal Article
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