Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-4-23
pubmed:abstractText
Hepatitis C virus (HCV) is one of the major causative agents of chronic liver disease with the potential for development of hepatocellular carcinoma. The putative core protein of the virus has many intriguing properties, including transcriptional regulation of cellular and unrelated viral promoters. To further characterize the transregulatory function, a number of chimeric constructs were made by fusion of the core gene to the DNA binding domain of the yeast transactivator factor GAL4. The fusion protein exhibited a repressor activity on the herpes simplex virus thymidine kinase promoter via the upstream GAL4 DNA binding sites. A structure /function analysis of HCV core mutants in the context of the GAL4 DNA binding domain revealed that the transcriptional repressor activity was located near the N-terminus (amino acids 26 85). Transcription was strongly inhibited upon transfer of this repressor domain to a heterologous activation domain, (3CGln) of Epstein Barr virus transcription factor EBNA3C. Results from this study suggest that the HCV core protein contains an overall repressor activity, and that the repressor domain is located near the N-terminus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0168-1702
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
211-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10082392-3T3 Cells, pubmed-meshheading:10082392-Animals, pubmed-meshheading:10082392-COS Cells, pubmed-meshheading:10082392-Chloramphenicol O-Acetyltransferase, pubmed-meshheading:10082392-DNA-Binding Proteins, pubmed-meshheading:10082392-Fungal Proteins, pubmed-meshheading:10082392-Gene Expression Regulation, pubmed-meshheading:10082392-Hepacivirus, pubmed-meshheading:10082392-Humans, pubmed-meshheading:10082392-Mice, pubmed-meshheading:10082392-Recombinant Fusion Proteins, pubmed-meshheading:10082392-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:10082392-Saccharomyces cerevisiae Proteins, pubmed-meshheading:10082392-Sequence Deletion, pubmed-meshheading:10082392-Transcription Factors, pubmed-meshheading:10082392-Tumor Cells, Cultured, pubmed-meshheading:10082392-Viral Core Proteins
pubmed:year
1999
pubmed:articleTitle
Functional analysis of a transrepressor domain in the hepatitis C virus core protein.
pubmed:affiliation
Department of Pathology, Saint Louis University, MO 63104, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't