Source:http://linkedlifedata.com/resource/pubmed/id/10082310
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1999-5-25
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pubmed:abstractText |
Flow cytometry of immunofluorescence and DNA content provides measures of cell-cycle-related gene expression (protein and/or epitope levels) for asynchronously growing cells. From these data, time-related expression through S phase can be directly measured. However, for G1, G2, and M phases, this information is unavailable. We present an objective method to model G1 and G2 kinetic expression from an estimate of a minimum biological unit of positive immunofluorescence derived from the distribution of specific immunofluorescence of mitotic cells.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0196-4763
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
284-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10082310-Cell Cycle,
pubmed-meshheading:10082310-Computer Simulation,
pubmed-meshheading:10082310-Cyclin B,
pubmed-meshheading:10082310-Cyclin B1,
pubmed-meshheading:10082310-Flow Cytometry,
pubmed-meshheading:10082310-Fluorescent Antibody Technique,
pubmed-meshheading:10082310-G1 Phase,
pubmed-meshheading:10082310-G2 Phase,
pubmed-meshheading:10082310-Gene Expression,
pubmed-meshheading:10082310-Humans,
pubmed-meshheading:10082310-Kinetics,
pubmed-meshheading:10082310-Male,
pubmed-meshheading:10082310-Mitosis,
pubmed-meshheading:10082310-Prostatic Neoplasms,
pubmed-meshheading:10082310-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Estimation of kinetic cell-cycle-related gene expression in G1 and G2 phases from immunofluorescence flow cytometry data.
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pubmed:affiliation |
Cancer Research Center, Case Western Reserve University, Cleveland, Ohio 44106-4944, USA. jwj@po.cwru.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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