Linked Life Data

10082078

Source:http://linkedlifedata.com/resource/pubmed/id/10082078

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-5-24
pubmed:abstractText
Characteristic autophosphorylation of calcium/ calmodulin-dependent protein kinase II (CaMKII) and its consequences have made this kinase an interesting target in studying the molecular pathway for important neuronal functions including learning and memory formation. In this article, we use immunoprecipitation and immunoblotting methods to detect changes in phosphorylation of CaMKII during memory formation in 1-day-old chicks trained in a single trial passive avoidance task. A 60-kDa protein has been immunoprecipitated from the chick brain with an anti-rabbit CaMKII antibody. This protein shows strong and specific immunoactivities with antibodies against the calmodulin binding site of CaMKII, and the N and C terminals of beta-CaMKII. Commercially available anti-phosphoserine and anti-phosphothreonine antibodies are shown to sensitively detect phosphorylation of purified CaMKII. The basal phosphorylation of CaMKII from the intermediate medial hyperstriatum ventrale (IMHV) and lobus parolfactorius (LPO) regions of the chick brain is shown to be largely right hemisphere-lateralized. When chicks are subjected to a passive avoidance training experience, a specific increase in CaMKII phosphorylation is induced in the IMHV and LPO of the left hemisphere from those chicks whose memory for the training experience is successfully retrieved. While this specific increase in CaMKII phosphorylation is seen in both the left IMHV and left LPO in short-term memory, it is detectable only in the left LPO associated with long-term memory retrieval. The present results provide evidence that in vivo changes in phosphorylation of CaMKII are associated specifically with processing of distinct memory stages, which take place in specific brain regions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
557-68
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10082078-3T3 Cells, pubmed-meshheading:10082078-Animals, pubmed-meshheading:10082078-Animals, Newborn, pubmed-meshheading:10082078-Avoidance Learning, pubmed-meshheading:10082078-Binding Sites, pubmed-meshheading:10082078-Blotting, Western, pubmed-meshheading:10082078-Brain, pubmed-meshheading:10082078-Calcium-Calmodulin-Dependent Protein Kinase Type 2, pubmed-meshheading:10082078-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:10082078-Chickens, pubmed-meshheading:10082078-Color Perception Tests, pubmed-meshheading:10082078-Cross Reactions, pubmed-meshheading:10082078-Discrimination Learning, pubmed-meshheading:10082078-Memory, pubmed-meshheading:10082078-Memory, Short-Term, pubmed-meshheading:10082078-Mice, pubmed-meshheading:10082078-Molecular Weight, pubmed-meshheading:10082078-Phosphorylation, pubmed-meshheading:10082078-Phosphoserine, pubmed-meshheading:10082078-Phosphothreonine, pubmed-meshheading:10082078-Precipitin Tests, pubmed-meshheading:10082078-Rats, pubmed-meshheading:10082078-Recombinant Proteins
pubmed:year
1999
pubmed:articleTitle
Changes in phosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaMKII) in processing of short-term and long-term memories after passive avoidance learning.
pubmed:affiliation
Department of Psychology, Monash University, Clayton, Victoria, Australia. wqzhao@helix.nih.gov
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't