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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-6-16
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pubmed:databankReference |
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pubmed:abstractText |
The ornithine transaminase (otaA) gene of Aspergillus nidulans has been cloned by transformation of the A. nidulans pro-ota- mutant strain with a cosmid gene library. The otaA gene contains two introns and potentially codes for a 453-aa-long protein. The deduced amino-acid sequence is homologous to known ornithine transaminases from Saccharomyces cerevisiae, Plasmodium falciparum, Vigna aconitifolia, rat, mouse and man, particularly in the pyridoxal phosphate-binding domain. The expression of the otaA gene is specifically induced by arginine, and is also under the control of nitrogen-metabolite and carbon-catabolite repression. Regulation of the gene occurs at both transcriptional and post-transcriptional levels. The promoter region of otaA contains putative AREA and CREA binding-sites. Fusion proteins containing AREA or CREA DNA-binding domains bind some of these sites. CREA binding-sites correspond very well to the CREA-binding consensus sequence which is SYGGRG. AREA binding-sites are composed of GATT sequences which are not typical binding sites for the GATA - binding family of transcription factors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0172-8083
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
118-26
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10079330-Amino Acid Sequence,
pubmed-meshheading:10079330-Aspergillus nidulans,
pubmed-meshheading:10079330-Base Sequence,
pubmed-meshheading:10079330-Binding Sites,
pubmed-meshheading:10079330-Cloning, Molecular,
pubmed-meshheading:10079330-DNA, Fungal,
pubmed-meshheading:10079330-Fungal Proteins,
pubmed-meshheading:10079330-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:10079330-Gene Expression Regulation, Fungal,
pubmed-meshheading:10079330-Genes, Fungal,
pubmed-meshheading:10079330-Molecular Sequence Data,
pubmed-meshheading:10079330-Ornithine-Oxo-Acid Transaminase,
pubmed-meshheading:10079330-Promoter Regions, Genetic,
pubmed-meshheading:10079330-Repressor Proteins,
pubmed-meshheading:10079330-Sequence Analysis, DNA,
pubmed-meshheading:10079330-Sequence Homology, Amino Acid,
pubmed-meshheading:10079330-Transcription, Genetic,
pubmed-meshheading:10079330-Transcription Factors
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pubmed:year |
1999
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pubmed:articleTitle |
Cloning, characterisation and regulation of the ornithine transaminase (otaA) gene of Aspergillus nidulans.
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pubmed:affiliation |
Department of Genetics, Warsaw University ul. Pawinskiego 5A, 02-106 Warsaw, Poland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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