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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-4-13
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pubmed:abstractText |
Reactive oxygen species (ROS) production in rat cerebellar granule cells in the presence of the excitotoxins N-methyl-d-aspartate (NMDA) and kainic acid (KA) and by the protein kinase C activator phorbol myristate acetate (PMA) was Ca2+-dependent and resulted in decreased cell viability. Exposure of stimulated cells to rotenone (a respiratory chain inhibitor) did not decrease ROS levels and did not affect short-term cell viability. In cells stimulated by NMDA and KA, exposure to indomethacin (a cyclooxygenase inhibitor) and nialamide (a monoamine oxidase inhibitor) caused a decrease in ROS levels and increased cell viability occurred in NMDA-treated cells. In contrast, PMA-stimulated neurons did not show decreased ROS levels when exposed to indomethacin and nialamide. These studies suggest that there is a multiplicity of routes for Ca2+-dependent ROS production in neurons but that ROS generation by cyclooxygenase and monoamine oxidase is not controlled by protein kinase C.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2',7'-dichlorofluorescein,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/Kainic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Monoamine Oxidase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/N-Methylaspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Nialamide,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Rotenone,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
256
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
320-4
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10079182-Animals,
pubmed-meshheading:10079182-Calcium,
pubmed-meshheading:10079182-Cell Survival,
pubmed-meshheading:10079182-Cells, Cultured,
pubmed-meshheading:10079182-Cerebellar Cortex,
pubmed-meshheading:10079182-Cyclooxygenase Inhibitors,
pubmed-meshheading:10079182-Electron Transport,
pubmed-meshheading:10079182-Flow Cytometry,
pubmed-meshheading:10079182-Fluoresceins,
pubmed-meshheading:10079182-Indomethacin,
pubmed-meshheading:10079182-Kainic Acid,
pubmed-meshheading:10079182-Monoamine Oxidase Inhibitors,
pubmed-meshheading:10079182-N-Methylaspartate,
pubmed-meshheading:10079182-Neurons,
pubmed-meshheading:10079182-Neurotoxins,
pubmed-meshheading:10079182-Nialamide,
pubmed-meshheading:10079182-Protein Kinase C,
pubmed-meshheading:10079182-Rats,
pubmed-meshheading:10079182-Rats, Sprague-Dawley,
pubmed-meshheading:10079182-Reactive Oxygen Species,
pubmed-meshheading:10079182-Rotenone,
pubmed-meshheading:10079182-Tetradecanoylphorbol Acetate
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pubmed:year |
1999
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pubmed:articleTitle |
Sources of reactive oxygen species production in excitotoxin- stimulated cerebellar granule cells.
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pubmed:affiliation |
Department of Biochemistry, International Biotechnological Center of M. V. Lomonosov Moscow State University, Moscow, 119899, Russia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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