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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-4-13
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pubmed:abstractText |
Epithelin/granulin growth factor is synthesized as a 593 amino acid precursor protein that contains 7.5 imperfectly conserved repeats of approximately 57 amino acids. Processed epithelin/granulin peptides have been isolated from vertebrate/invertebrate species and are growth factors implicated in epithelial and haemic cell function. Here they are identified as Human Immunodeficiency Virus (HIV) Tat binding proteins using the yeast two-hybrid assay. Intracellularly in yeast, mutation of selected cysteines in an epithelin/granulin dimeric repeat caused loss of binding to Tat exon 1. In vitro binding of HIV-1 and HIV-2 Tat to epithelin/granulin dimeric and monomeric repeats was also observed by GST-glutathione bead "pulldown" assays. Because Tat is actively secreted from HIV-infected cells and has been shown to serve as a mitogenic factor for angiogenesis and for Kaposi-like cells, our observations suggest that epithelin/granulin growth factors may function as biologically important extracellular Tat co-factors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/granulin 4,
http://linkedlifedata.com/resource/pubmed/chemical/serine-arginine-rich splicing...,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
256
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
299-306
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10079180-Amino Acid Sequence,
pubmed-meshheading:10079180-Amino Acid Substitution,
pubmed-meshheading:10079180-Binding Sites,
pubmed-meshheading:10079180-Conserved Sequence,
pubmed-meshheading:10079180-Cysteine,
pubmed-meshheading:10079180-Exons,
pubmed-meshheading:10079180-Gene Library,
pubmed-meshheading:10079180-Gene Products, tat,
pubmed-meshheading:10079180-Growth Substances,
pubmed-meshheading:10079180-HIV-1,
pubmed-meshheading:10079180-HIV-2,
pubmed-meshheading:10079180-Humans,
pubmed-meshheading:10079180-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:10079180-Leukocytes, Mononuclear,
pubmed-meshheading:10079180-Molecular Sequence Data,
pubmed-meshheading:10079180-Nuclear Proteins,
pubmed-meshheading:10079180-Protein Binding,
pubmed-meshheading:10079180-RNA, Messenger,
pubmed-meshheading:10079180-RNA-Binding Proteins,
pubmed-meshheading:10079180-Recombinant Fusion Proteins,
pubmed-meshheading:10079180-Saccharomyces cerevisiae,
pubmed-meshheading:10079180-Sequence Deletion,
pubmed-meshheading:10079180-Tumor Cells, Cultured,
pubmed-meshheading:10079180-Viral Proteins,
pubmed-meshheading:10079180-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1999
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pubmed:articleTitle |
Epithelin/granulin growth factors: extracellular cofactors for HIV-1 and HIV-2 Tat proteins.
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pubmed:affiliation |
Department of Biological Sciences, George Washington University, 332 Lisner Hall, 2023 G Street N.W., Washington, DC 20052, USA.
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pubmed:publicationType |
Journal Article
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