Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1999-4-15
pubmed:abstractText
The formation of prostaglandins requires the catalytic activity of cyclooxygenase (COX) which converts arachidonic acid to the prostaglandin endoperoxide PGH2, from which all other prostaglandins are formed. COX-2 is the highly inducible isozyme of COX which is responsible for much of the prostaglandin production in inflammation and is a key factor in colon carcinogenesis. Because COX-2 activity can be rate-limiting in prostaglandin formation, COX-2 expression must be regulated tightly. Numerous factors, including mitogens, tumor promoters, and cytokines have been found to stimulate the transcription of COX-2. We show that fatty acids, prostaglandins, and non-steroidal anti-inflammatory drugs, compounds that are substrates, products, and inhibitors, respectively, of COX enzymatic activity, also increase its expression. These compounds are members of a heterogeneous group of compounds known as peroxisome proliferators, and the prototypical peroxisome proliferator, WY-14, 643, also enhanced COX-2 expression. We demonstrate that these compounds increase COX-2 transcription, and we identify a region of the COX-2 promoter containing a peroxisome proliferator response element that is responsible for the enhancement of COX-2 expression seen with these compounds.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
274
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8328-34
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10075740-Animals, pubmed-meshheading:10075740-Anti-Inflammatory Agents, Non-Steroidal, pubmed-meshheading:10075740-Cyclooxygenase 2, pubmed-meshheading:10075740-Enzyme Induction, pubmed-meshheading:10075740-Epithelial Cells, pubmed-meshheading:10075740-Fatty Acids, pubmed-meshheading:10075740-Gene Expression Regulation, Enzymologic, pubmed-meshheading:10075740-Goats, pubmed-meshheading:10075740-Humans, pubmed-meshheading:10075740-Isoenzymes, pubmed-meshheading:10075740-Membrane Proteins, pubmed-meshheading:10075740-Mice, pubmed-meshheading:10075740-Peroxisome Proliferators, pubmed-meshheading:10075740-Prostaglandin-Endoperoxide Synthases, pubmed-meshheading:10075740-Prostaglandins, pubmed-meshheading:10075740-Pyrimidines, pubmed-meshheading:10075740-Transcription, Genetic
pubmed:year
1999
pubmed:articleTitle
Peroxisome proliferators enhance cyclooxygenase-2 expression in epithelial cells.
pubmed:affiliation
Eccles Program in Human Molecular Biology and Genetics, the Huntsman Cancer Institute, the University of Utah, Salt Lake City, Utah 84112, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.