Source:http://linkedlifedata.com/resource/pubmed/id/10075648
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
1999-4-15
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pubmed:abstractText |
We report that the heterotrimeric transcription factor NFY or "CAAT-binding factor" binds the -60 region of the human H ferritin promoter, the B site. DNA binding analysis with specific antibodies demonstrates that NFY/B/C subunits tightly bind this site and that NFY/C subunit is masked in vivo by binding with other protein(s). NFY binds the co-activator p300. Specifically, the NFY/B subunit interacts with the central segment of p300 in vivo and in vitro. cAMP substantially increases the formation of the NFY.p300 complex. Taken together these data provide a general model of cAMP induction of non-CRE-containing promoters and suggest that the NFY-B.p300 complex is located at the 5' end of the promoter and the NFY-B.C. TFIIB on the 3' end toward the transcription start site.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ferritins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7623-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10075648-Binding Sites,
pubmed-meshheading:10075648-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:10075648-DNA-Binding Proteins,
pubmed-meshheading:10075648-Ferritins,
pubmed-meshheading:10075648-HeLa Cells,
pubmed-meshheading:10075648-Humans,
pubmed-meshheading:10075648-Nuclear Proteins,
pubmed-meshheading:10075648-Promoter Regions, Genetic,
pubmed-meshheading:10075648-Protein Conformation,
pubmed-meshheading:10075648-Trans-Activators
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pubmed:year |
1999
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pubmed:articleTitle |
The B subunit of the CAAT-binding factor NFY binds the central segment of the Co-activator p300.
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pubmed:affiliation |
Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli 'Federico II', Via S. Pansini 5, I-80131 Napoli, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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