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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1999-4-2
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pubmed:abstractText |
The cytokine profile of atherosclerotic aortas from apoE-deficient mice was assessed by reverse transcriptase-polymerase chain reaction. The results clearly showed that the expression of mRNA for IL-12p40 was evident in aortas from 3-month-old apoE-deficient mice. The mRNA for IL-10 was detected in aorta from these mice at the age of 6 months, indicating that expression of IL-12 is earlier than that of IL-10 in these animals. Concurrent with IL-12p40, the mRNA for the T-cell cytokine IFN-gamma, but not IL-4, was detected in aortas of mice at young and old ages. Both in situ hybridization and immunostaining further demonstrated the localization of IL-12 in macrophages of atherosclerotic lesions. Immunohistochemistry also demonstrated the expression of costimulatory molecules B7-1 and B7-2 in macrophages, suggesting that activation of T lymphocytes by macrophages may occur via surface antigens in lesions. When the immunoglobulin isotype of the antioxidized LDL antibodies in sera of apoE-deficient mice was determined, it revealed that both IgM and IgG were present. Furthermore, IgG2a is predominant and comprises approximately 50% of the antioxidized LDL IgG in sera from young mice (3 months), but decreased to lower levels (35%) in older mice (6 months). Daily administration of IL-12 led to an increase in serum levels of antioxidized LDL antibodies and accelerated atherosclerosis in young apoE-deficient mice compared with control mice injected with PBS alone. Taken together, these data suggest that IL-12 plays an active role in regulating the immune response during the early phase of atherosclerosis in apoE-deficient mice.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins E,
http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/oxidized low density lipoprotein
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1079-5642
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
734-42
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10073981-Animals,
pubmed-meshheading:10073981-Antigens, CD,
pubmed-meshheading:10073981-Antigens, CD80,
pubmed-meshheading:10073981-Antigens, CD86,
pubmed-meshheading:10073981-Apolipoproteins E,
pubmed-meshheading:10073981-Arteriosclerosis,
pubmed-meshheading:10073981-Cholesterol, LDL,
pubmed-meshheading:10073981-DNA Probes,
pubmed-meshheading:10073981-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10073981-Foam Cells,
pubmed-meshheading:10073981-Gene Expression,
pubmed-meshheading:10073981-Immunoglobulin G,
pubmed-meshheading:10073981-Immunoglobulin M,
pubmed-meshheading:10073981-Interleukin-12,
pubmed-meshheading:10073981-Lipoproteins, LDL,
pubmed-meshheading:10073981-Membrane Glycoproteins,
pubmed-meshheading:10073981-Mice,
pubmed-meshheading:10073981-Mice, Inbred C57BL,
pubmed-meshheading:10073981-Mice, Mutant Strains,
pubmed-meshheading:10073981-RNA, Messenger,
pubmed-meshheading:10073981-Recombinant Proteins,
pubmed-meshheading:10073981-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10073981-T-Lymphocytes
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pubmed:year |
1999
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pubmed:articleTitle |
The role of interleukin 12 in the development of atherosclerosis in ApoE-deficient mice.
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pubmed:affiliation |
Division of Cardiovascular Research, Institute of Biomedical Sciences, Academia Sinica, Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, Taiwan, R.O.C.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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