Linked Life Data

10073966

Source:http://linkedlifedata.com/resource/pubmed/id/10073966

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-4-2
pubmed:abstractText
The effect of the expression of human hepatic lipase (HL) or human apoE on plasma lipoproteins in transgenic rabbits in response to dietary cholesterol was compared with the response of nontransgenic control rabbits. Supplementation of a chow diet with 0.3% cholesterol and 3.0% soybean oil for 10 weeks resulted in markedly increased levels of plasma cholesterol and VLDL and IDL in control rabbits as expected. Expression of either HL or apoE reduced plasma cholesterol response by 75% and 60%, respectively. The HL transgenic rabbits had substantial reductions in medium and small VLDL and IDL fractions but not in larger VLDL. LDL levels were also reduced, with a shift from larger, more buoyant to smaller, denser particles. In contrast, apoE transgenic rabbits had a marked reduction in the levels of large VLDLs, with a selective accumulation of IDLs and large buoyant LDLs. Combined expression of apoE and HL led to dramatic reductions of total cholesterol (85% versus controls) and of total VLDL+IDL+LDL (87% versus controls). HDL subclasses were remodeled by the expression of either transgene and accompanied by a decrease in HDL cholesterol compared with controls. HL expression reduced all subclasses except for HDL2b and HDL2a, and expression of apoE reduced large HDL1 and HDL2b. Extreme HDL reductions (92% versus controls) were observed in the combined HL+apoE transgenic rabbits. These results demonstrate that human HL and apoE have complementary and synergistic functions in plasma cholesterol and lipoprotein metabolism.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1079-5642
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
625-32
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10073966-Animals, pubmed-meshheading:10073966-Animals, Genetically Modified, pubmed-meshheading:10073966-Apolipoproteins B, pubmed-meshheading:10073966-Apolipoproteins E, pubmed-meshheading:10073966-Cholesterol, pubmed-meshheading:10073966-Cholesterol, Dietary, pubmed-meshheading:10073966-Cholesterol, HDL, pubmed-meshheading:10073966-Cholesterol, VLDL, pubmed-meshheading:10073966-Diet, Atherogenic, pubmed-meshheading:10073966-Gene Expression, pubmed-meshheading:10073966-Humans, pubmed-meshheading:10073966-Hypercholesterolemia, pubmed-meshheading:10073966-Lipase, pubmed-meshheading:10073966-Lipoproteins, pubmed-meshheading:10073966-Liver, pubmed-meshheading:10073966-Male, pubmed-meshheading:10073966-Particle Size, pubmed-meshheading:10073966-Rabbits, pubmed-meshheading:10073966-Transgenes
pubmed:year
1999
pubmed:articleTitle
Overexpression of human hepatic lipase and ApoE in transgenic rabbits attenuates response to dietary cholesterol and alters lipoprotein subclass distributions.
pubmed:affiliation
Istituto di Medicina Interna e Geriatria, Universitá degli Studi di Palermo, Italy.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't