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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1999-3-25
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pubmed:abstractText |
LEF-3 and P143 are two of six proteins encoded by the Autographa californica multinucleocapsid nucleopolyhedrovirus genome which are required for DNA replication in transient replication assays. LEF-3 has the properties of an ssDNA-binding protein and P143 exhibits amino acid sequence homology to helicases. In this report, the interaction of LEF-3 and P143 was studied by yeast two-hybrid and immunoaffinity analyses. Using the yeast two-hybrid system, the interaction domain of LEF-3 (385 aa) was mapped to amino acids between positions 1 and 165. Deletion analysis of P143 failed to reveal an interaction domain, suggesting that there were either multiple interaction domains or that the deletions disrupted the secondary structures required for the interaction between LEF-3 and P143.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
493-500
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10073712-Base Sequence,
pubmed-meshheading:10073712-DNA Helicases,
pubmed-meshheading:10073712-DNA Primers,
pubmed-meshheading:10073712-DNA Replication,
pubmed-meshheading:10073712-DNA-Binding Proteins,
pubmed-meshheading:10073712-Escherichia coli,
pubmed-meshheading:10073712-Immediate-Early Proteins,
pubmed-meshheading:10073712-Nucleopolyhedrovirus,
pubmed-meshheading:10073712-Protein Structure, Secondary,
pubmed-meshheading:10073712-Saccharomyces cerevisiae,
pubmed-meshheading:10073712-Sequence Deletion,
pubmed-meshheading:10073712-Trans-Activators,
pubmed-meshheading:10073712-Viral Proteins
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pubmed:year |
1999
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pubmed:articleTitle |
Characterization of the interaction between the baculovirus ssDNA-binding protein (LEF-3) and putative helicase (P143).
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pubmed:affiliation |
Department of Microbiology, Oregon State University, Corvallis 97331-3804, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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