Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1999-4-13
pubmed:databankReference
pubmed:abstractText
The kelch family of proteins is defined by a 50 amino-acid repeat that has been shown to associate with actin. Here we describe the cloning and initial characterization of IPP, a novel human gene that predicts a kelch family protein homologous to the mouse Ipp gene, a previously described kelch family member. A 3kb IPP cDNA clone was isolated from a human placenta library using a probe derived from Ipp. Restriction mapping and Southern blot analysis show that IPP comprises eight exons spanning more than 47kb of genomic DNA. Fluorescence in situ hybridization maps the gene to chromosome 1p32-1p34. Northern blot analysis reveals transcripts of 1.4, 2.2, 5. 0, and 7.3kb. The 1.4 and 2.2kb messages are found exclusively in testis, while the 5.0 and 7.3kb messages are expressed at varying levels in ovary, placenta, small intestine, spleen, testis, and thymus. The IPP cDNA clone contains a 1752bp open reading frame that predicts a 584 amino-acid, 66kDa protein. Sequence analysis indicates an N-terminal POZ protein-protein interaction domain and a C-terminal kelch repeat domain consisting of six tandemly arranged repeats. Cosedimentation assays performed with these domains expressed as glutathione S-transferase fusion proteins demonstrate an actin-binding activity mediated specifically by the kelch repeat domain of IPP.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
228
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-83
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10072760-Actins, pubmed-meshheading:10072760-Amino Acid Sequence, pubmed-meshheading:10072760-Base Sequence, pubmed-meshheading:10072760-Binding Sites, pubmed-meshheading:10072760-Blotting, Northern, pubmed-meshheading:10072760-Carrier Proteins, pubmed-meshheading:10072760-Chromosome Mapping, pubmed-meshheading:10072760-Chromosomes, Human, Pair 1, pubmed-meshheading:10072760-Cytoskeletal Proteins, pubmed-meshheading:10072760-DNA, pubmed-meshheading:10072760-DNA, Complementary, pubmed-meshheading:10072760-Female, pubmed-meshheading:10072760-Genes, pubmed-meshheading:10072760-HeLa Cells, pubmed-meshheading:10072760-Humans, pubmed-meshheading:10072760-Male, pubmed-meshheading:10072760-Microfilament Proteins, pubmed-meshheading:10072760-Molecular Sequence Data, pubmed-meshheading:10072760-Pregnancy Proteins, pubmed-meshheading:10072760-Protein Binding, pubmed-meshheading:10072760-RNA, Messenger, pubmed-meshheading:10072760-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:10072760-Sequence Analysis, DNA, pubmed-meshheading:10072760-Sequence Homology, Amino Acid, pubmed-meshheading:10072760-Tissue Distribution
pubmed:year
1999
pubmed:articleTitle
Isolation and characterization of IPP, a novel human gene encoding an actin-binding, kelch-like protein.
pubmed:affiliation
The Johns Hopkins University, Department of Biology, 3400 North Charles Street, Baltimore, MD 21218, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.