Source:http://linkedlifedata.com/resource/pubmed/id/10072526
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1999-4-14
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pubmed:databankReference | |
pubmed:abstractText |
The murine Ig heavy chain (IgH) 3' regulatory region contains four enhancers: hs3A, hs1,2, hs3B, and hs4. Various studies have suggested a role for these enhancers in regulating IgH expression and class switching. Here we assess the role of hs3A and hs1,2 in these processes by exploiting a naturally occurring deletion of these enhancers from the expressed, C57BL/6 allele of the F1 pre-B cell line, 70Z/3. Equivalent mu expression in 70Z/3 and 18-81 (which has an intact 3' region) indicated that hs3A and hs1,2 were not essential for mu expression at the pre-B cell stage. To further examine the role of hs3A and hs1,2 in IgH function at the plasma cell stage, we fused 70Z/3 with the plasmacytoma NSO. Electromobility shift assay analysis of the 70Z/3-NSO hybrids revealed a transcription factor complement conducive to the activation of the 3' enhancers. Despite the lack of enhancers, hs3A and hs1,2, the level of mu RNA and protein in the 70Z/3-NSO fusion hybrids was substantially elevated relative to its pre-B parent and comparable with that observed in a number of mu-producing spleen cell hybridomas. Additionally, ELISAspot assays showed that the 70Z/3-NSO hybrid underwent spontaneous class switching in culture to IgG1 at a frequency comparable with that of most hybridomas. These results indicate that hs3A and hs1,2 are not essential for high levels of IgH expression or for spontaneous class switching in a plasma cell line.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2791-803
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10072526-Alleles,
pubmed-meshheading:10072526-Amino Acid Sequence,
pubmed-meshheading:10072526-Animals,
pubmed-meshheading:10072526-Base Sequence,
pubmed-meshheading:10072526-Deoxyribonuclease I,
pubmed-meshheading:10072526-Enhancer Elements, Genetic,
pubmed-meshheading:10072526-Genes, Immunoglobulin,
pubmed-meshheading:10072526-Immunoglobulin Class Switching,
pubmed-meshheading:10072526-Immunoglobulin Heavy Chains,
pubmed-meshheading:10072526-Mice,
pubmed-meshheading:10072526-Mice, Inbred BALB C,
pubmed-meshheading:10072526-Mice, Inbred C57BL,
pubmed-meshheading:10072526-Mice, Inbred DBA,
pubmed-meshheading:10072526-Molecular Sequence Data
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pubmed:year |
1999
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pubmed:articleTitle |
Ig heavy chain expression and class switching in vitro from an allele lacking the 3' enhancers DNase I-hypersensitive hs3A and hs1,2.
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pubmed:affiliation |
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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