Linked Life Data

10069862

Source:http://linkedlifedata.com/resource/pubmed/id/10069862

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-5-3
pubmed:abstractText
Campylobacter jejuni is a significant cause of food-borne diseases in humans. The bacterium is considered a commensal organism in chickens, and it can heavily colonize chickens without causing inflammation. Poultry may be the major reservoir for the human infection in developed countries. Here we show that an outer-membrane protein extract prepared from the bacteria caused apoptosis of chicken lymphocytes detected in vitro with the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay that preferentially labels individual apoptotic cells. Blood- and spleen-lymphocytes from different-aged chickens displayed a significantly greater percentage of apoptotic cells after culture with the outer-membrane proteins from C. jejuni than controls treated with phosphate-buffered saline, chicken ovalbumin, or outer-membrane proteins prepared from E. coli strain BL21. The C. jejuni extract also produced apoptosis of chicken lymphoblastoid tumor cell lines. Apoptosis was blocked by pretreating the extract with proteinase K or antiserum against outer-membrane proteins. The results suggest that C. jejuni may be capable of achieving immune avoidance in chickens by causing apoptosis of lymphocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0343-8651
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
244-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Apoptotic effect of outer-membrane proteins from Campylobacter jejuni on chicken lymphocytes.
pubmed:affiliation
Poultry Microbiological Safety Research Unit, Russell Agricultural Research Center, U.S. Department of Agriculture, Agricultural Research Service, Athens, GA 30604.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.