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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-3-11
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pubmed:abstractText |
Evidence is accumulating which indicates that cyclooxygenase-2 (COX-2) is involved in the pathogenesis of colorectal cancer. We evaluated the expression of COX-2 in replication error-positive (RER) colon cancers, colon cancers metastatic to liver and azoxymethane (AOM)-induced rat colonic tumors. Immunohistochemistry showed that COX-2 was low to undetectable in normal human mucosa, but abundant in the RER adenocarcinomas we examined. COX-2 immunoreactivity in metastatic colon cancers was less abundant, but clearly detectable. In the colon of AOM-treated rats, COX-2 protein was not detectable in normal mucosa, but present in most of the epithelial cells comprising the tumors. The TGF-beta1 staining pattern in these human and rat tumors was similar to that observed for COX-2. The role of TGF-beta in RER adenocarcinomas is complex because of the increased mutation rate of TGF-beta type II receptors. Northern analysis showed abundant TGF-beta1 mRNA in AOM-induced tumors, but not in paired mucosa. TGF-beta1 induced the expression of COX-2 mRNA and protein in intestinal epithelial cells (IEC-6). Chronic TGF-beta1 treatment caused a TGF-beta-dependent overexpression of COX-2 in rat intestinal epithelial cells (RIE-1). TGF-beta1 may regulate COX-2 expression during the colonic adenoma to carcinoma sequence.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Azoxymethane,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/Ptgs1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0143-3334
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pubmed:author |
pubmed-author:AramandlaRR,
pubmed-author:BeauchampR DRD,
pubmed-author:DuBoisR NRN,
pubmed-author:GroganLL,
pubmed-author:HawkEE,
pubmed-author:KirschI RIR,
pubmed-author:LubetR ARA,
pubmed-author:PereiraM AMA,
pubmed-author:ShawBB,
pubmed-author:ShengHH,
pubmed-author:WashingtonM KMK
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
185-91
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10069452-Animals,
pubmed-meshheading:10069452-Azoxymethane,
pubmed-meshheading:10069452-Carcinogens,
pubmed-meshheading:10069452-Colon,
pubmed-meshheading:10069452-Colonic Neoplasms,
pubmed-meshheading:10069452-Cyclooxygenase 1,
pubmed-meshheading:10069452-Cyclooxygenase 2,
pubmed-meshheading:10069452-Enzyme Induction,
pubmed-meshheading:10069452-Epithelial Cells,
pubmed-meshheading:10069452-Humans,
pubmed-meshheading:10069452-Immunohistochemistry,
pubmed-meshheading:10069452-Intestinal Mucosa,
pubmed-meshheading:10069452-Isoenzymes,
pubmed-meshheading:10069452-Liver Neoplasms,
pubmed-meshheading:10069452-Male,
pubmed-meshheading:10069452-Membrane Proteins,
pubmed-meshheading:10069452-Neoplasm Proteins,
pubmed-meshheading:10069452-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:10069452-RNA, Messenger,
pubmed-meshheading:10069452-Rats,
pubmed-meshheading:10069452-Rats, Inbred F344,
pubmed-meshheading:10069452-Transforming Growth Factor beta
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pubmed:year |
1999
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pubmed:articleTitle |
Coordinate regulation of cyclooxygenase-2 and TGF-beta1 in replication error-positive colon cancer and azoxymethane-induced rat colonic tumors.
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pubmed:affiliation |
Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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