Linked Life Data

10068473

Source:http://linkedlifedata.com/resource/pubmed/id/10068473

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-5-3
pubmed:databankReference
pubmed:abstractText
This work concerns the expression of two transcription factors during the development of the sea urchin Strongylocentrotus purpuratus: SpNot, the orthologue of the vertebrate Not gene, and SpBra, the orthologue of the vertebrate Brachyury gene. SpNot transcripts are detected by in situ hybridization in the vegetal plate at the mesenchyme-blastula stage. Later the gene is expressed in the secondary mesenchyme, but expression is no longer detectable after gastrulation. SpNot is upregulated during larval development, in the invaginating vestibule of the adult rudiment. Transcripts are also found in several larva-specific tissues, including the epaulets, blastocoelar cells, and pigment cells. SpBra also displays a discontinuous pattern of expression. Much like SpNot, this gene is expressed during embryogenesis in the embryonic vegetal plate and secondary mesenchyme founder cells, and expression is then extinguished. The gene is upregulated over a week later in the feeding larva, in the vestibule of the adult rudiment. In contrast to SpNot, SpBra is also expressed in the mesoderm of both left and right hydrocoels, and it is not expressed in any larva-specific tissues. We compare the spatial expression profile determined in this study with that of the orthologous Brachyury gene in an indirectly developing enteropneust hemichordate, a representative of the sister group to the echinoderms within the deuterostomes. These observations illuminate the genetic basis underlying the process of maximal indirect development in basal deuterostomes. Finally, Brachyury appears to be an excellent marker for the progeny of the set-aside cells of the sea urchin embryo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0012-1606
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
207
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
419-31
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10068473-Amino Acid Sequence, pubmed-meshheading:10068473-Animals, pubmed-meshheading:10068473-Base Sequence, pubmed-meshheading:10068473-Cloning, Molecular, pubmed-meshheading:10068473-DNA-Binding Proteins, pubmed-meshheading:10068473-Embryonic Development, pubmed-meshheading:10068473-Evolution, Molecular, pubmed-meshheading:10068473-Fetal Proteins, pubmed-meshheading:10068473-Gene Expression Regulation, Developmental, pubmed-meshheading:10068473-Mesoderm, pubmed-meshheading:10068473-Molecular Sequence Data, pubmed-meshheading:10068473-Nuclear Receptor Subfamily 4, Group A, Member 2, pubmed-meshheading:10068473-RNA, Messenger, pubmed-meshheading:10068473-Sea Urchins, pubmed-meshheading:10068473-Sequence Alignment, pubmed-meshheading:10068473-Sequence Analysis, DNA, pubmed-meshheading:10068473-T-Box Domain Proteins, pubmed-meshheading:10068473-Transcription Factors
pubmed:year
1999
pubmed:articleTitle
Expression pattern of Brachyury and Not in the sea urchin: comparative implications for the origins of mesoderm in the basal deuterostomes.
pubmed:affiliation
Division of Biology, California Institute of Technology, Pasadena, California, 91125, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.