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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-4-13
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pubmed:abstractText |
Laminin is a potent stimulator of neurite outgrowth in rat pheochromocytoma (PC12) cells. Here, we investigated the role of protein kinase C (PKC) in the mechanism of laminin-mediated neurite outgrowth in PC12 cells. Phorbol ester activators of PKC have been shown to have divergent effects on laminin-mediated neurite outgrowth. Therefore, we tested the effect of the non-phorbol PKC activator, indolactam V. At 1.0 microM indolactam V inhibited laminin-mediated neurite outgrowth by 85%. Further, the PKC inhibitor H7 blocked the inhibitory effect of indolactam V on laminin-mediated neurite outgrowth. Direct measurement of protein kinase C activity in the soluble (cytosolic) and particulate (membrane) fractions of PC12 cells showed that laminin failed to alter protein kinase C activity. These data demonstrate that PKC activation inhibits laminin-mediated neurite outgrowth and that laminin does not activate PKC in PC12 cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
256
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
98-103
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10066430-1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine,
pubmed-meshheading:10066430-Animals,
pubmed-meshheading:10066430-Cell Membrane,
pubmed-meshheading:10066430-Cell Size,
pubmed-meshheading:10066430-Cell Survival,
pubmed-meshheading:10066430-Cytoplasm,
pubmed-meshheading:10066430-Enzyme Activation,
pubmed-meshheading:10066430-Indoles,
pubmed-meshheading:10066430-Insulin,
pubmed-meshheading:10066430-Lactams,
pubmed-meshheading:10066430-Laminin,
pubmed-meshheading:10066430-Neurites,
pubmed-meshheading:10066430-Oxazoles,
pubmed-meshheading:10066430-PC12 Cells,
pubmed-meshheading:10066430-Phosphoprotein Phosphatases,
pubmed-meshheading:10066430-Protein Kinase C,
pubmed-meshheading:10066430-Rats,
pubmed-meshheading:10066430-Signal Transduction,
pubmed-meshheading:10066430-Stereoisomerism,
pubmed-meshheading:10066430-Time Factors
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pubmed:year |
1999
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pubmed:articleTitle |
The role of protein kinase C in laminin-mediated neurite outgrowth.
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pubmed:affiliation |
Department of Biology, Adelphi University, Garden City, New York, 11530, USA.
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pubmed:publicationType |
Journal Article
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