Linked Life Data

10064880

Source:http://linkedlifedata.com/resource/pubmed/id/10064880

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1999-4-7
pubmed:abstractText
MAP5, a microtubule-associated protein present in immature neurons, was found to be expressed in the embryonic mouse telencephalic ventricular zone (VZ). Since the VZ contains proliferating neuroblasts, the source of most of the neurons of the cerebral cortex, this observation raised the possibility that MAP5 is expressed by proliferating neuronal progenitors. MAP5-positive mitotic cells were observed at the ventricular surface, a finding consistent with progenitors expressing MAP5 prior to their last division. This possibility was investigated using dissociated, cortical cells in vitro by measuring the expression of MAP5 and the neuroepithelial marker nestin, together with the incorporation of bromodeoxyuridine (BrdU), a thymidine analogue that labels the DNA of proliferating cells in the S-phase of the cell cycle. All of the proliferating cells expressed nestin. A population of MAP5-positive cells was also found to incorporate BrdU; some cells expressed MAP5 within 30 min of BrdU labeling. The results suggest that uncommitted neuroblasts express only nestin, with expression of MAP5 occurring near the time the cell commits to become a postmitotic neuron after the next cell division. Subsequently, cells expressing both MAP5 and nestin leave the cell cycle and exit the VZ, lose nestin, and differentiate into neurons. Since some cells expressed MAP5 during or shortly after S-phase but before mitosis, MAP5 may be the earliest marker to identify neuronal progenitors that will become post-mitotic neurons following their next mitosis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0165-3806
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Published by Elsevier Science B.V.
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
113
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-13
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
MAP5 expression in proliferating neuroblasts.
pubmed:affiliation
Department of Physiology, University of Maryland School of Medicine, 655 W. Baltimore Street, Baltimore, MD 21201, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't