Linked Life Data

10064798

Source:http://linkedlifedata.com/resource/pubmed/id/10064798

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-4-22
pubmed:abstractText
In the paracervical ganglion (PCG) of the rat, double-labelling immunofluorescence for catecholamine-synthesizing enzymes and HPLC measurement of catecholamine contents were first performed to evaluate whether intraganglionic small intensely fluorescent (SIF) cells are capable of synthesizing adrenaline. Immunolabelling for tyrosine hydroxylase (TH), dopamine beta-hydroxylase and phenylethanolamine-N-methyl transferase (PNMT) occurred in all SIF cells of the PCG, thus demonstrating the presence of all the enzymes required for adrenaline biosynthesis. Adrenaline levels were undetectable in the PCG but to test the hypothesis that PNMT is active in SIF cells, catecholamines were measured in ganglia of rats pretreated with pargyline, an inhibitor of the monoamine oxidase, the major enzyme involved in the catecholamine degradation. Pargyline treatment increased adrenaline levels in the PCG, thus demonstrating that SIF cells are capable of adrenaline synthesis. The undetectable levels of adrenaline in the PCG of untreated rats suggested a slow rate of biosynthesis of adrenaline in the ganglion. Furthermore, the use of double-labelling showed that SIF cells of the PCG were stained for muscarinic receptors and were approached by varicose ChAT-immunoreactive nerve fibres. Nerve fibres immunoreactive for ChAT were also observed associated with nerve cell bodies of ganglion neurones. Following deafferentation of the PCG, the ChAT-immunoreactive nerve fibres surrounding nerve cell bodies totally disappeared indicating their preganglionic origin, while those associated with SIF cells did not degenerate, which demonstrate that they derived from intraganglionic cholinergic neurones. Taken together, the results show that adrenaline may be a transmitter for SIF cells in the PCG and suggest that cholinergic neurones of the parasympathetic division of the PCG can modulate the SIF cell activity through the activation of muscarinic receptors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-8993
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Elsevier Science B.V.
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
821
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
141-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:10064798-Afferent Pathways, pubmed-meshheading:10064798-Animals, pubmed-meshheading:10064798-Autonomic Fibers, Postganglionic, pubmed-meshheading:10064798-Cell Line, pubmed-meshheading:10064798-Cell Size, pubmed-meshheading:10064798-Choline O-Acetyltransferase, pubmed-meshheading:10064798-Dopamine beta-Hydroxylase, pubmed-meshheading:10064798-Epinephrine, pubmed-meshheading:10064798-Female, pubmed-meshheading:10064798-Fluorescent Antibody Technique, pubmed-meshheading:10064798-Ganglia, Sympathetic, pubmed-meshheading:10064798-Immunohistochemistry, pubmed-meshheading:10064798-Neurons, pubmed-meshheading:10064798-Phenylethanolamine N-Methyltransferase, pubmed-meshheading:10064798-Rats, pubmed-meshheading:10064798-Rats, Sprague-Dawley, pubmed-meshheading:10064798-Receptors, Muscarinic, pubmed-meshheading:10064798-Tyrosine 3-Monooxygenase
pubmed:year
1999
pubmed:articleTitle
Small intensely fluorescent cells of the rat paracervical ganglion synthesize adrenaline, receive afferent innervation from postganglionic cholinergic neurones, and contain muscarinic receptors.
pubmed:affiliation
Laboratoire de Neurobiologie des Fonctions Végétatives, INRA, F-78352 Jouy-en-Josas Cedex, France.
pubmed:publicationType
Journal Article