Source:http://linkedlifedata.com/resource/pubmed/id/10064084
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1999-3-16
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pubmed:abstractText |
Thymectomy at day 3 of life (d3Tx) results in the development of organ-specific autoimmunity. We have recently shown that d3Tx BALB/c mice which develop autoimmune gastritis contain CD4+ T cells specific for the gastric parietal cell proton pump, H/K ATPase. Here, we demonstrate that freshly explanted gastric lymph node (LN) cells from d3Tx mice react significantly to the H/K ATPase alpha chain, but only marginally to the beta chain. Two H/K ATPase-reactive T cell lines were derived from the gastric LN of d3Tx mice. Both are CD4+, TCR alpha/beta-, and I-Ad restricted, and recognize distinct peptides from the H/K ATPase alpha chain. One cell line secretes Th1 and the other Th2 cytokines, but both are equally potent in inducing gastritis with distinct profiles of cellular infiltration in nu/nu recipient animals. Neither of the cell lines induced disease in normal BALB/c recipients and transfer of disease to nu/nu recipients was blocked by co-transfer of normal BALB/c spleen cells containing CD4+ CD25+ cells. Although CD4+ CD25+ T cells are thought to emigrate from the thymus after day 3 of life, they could be identified in LN of 2-day-old animals. The capacity of CD4+ CD25+ T cells to abrogate the pathogenic activity in vivo of both activated Th1/Th2 lines strongly suggests that this suppressor T cell population may have a therapeutic role in other models of established autoimmunity. The availability of well-characterized lines of autoantigen-specific T cells should greatly facilitate the analysis of the mechanism of action and target of the CD4+ CD25+ immunoregulatory cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-2980
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
669-77
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:10064084-Animals,
pubmed-meshheading:10064084-Autoantigens,
pubmed-meshheading:10064084-Autoimmune Diseases,
pubmed-meshheading:10064084-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10064084-Cytotoxicity, Immunologic,
pubmed-meshheading:10064084-Gastritis,
pubmed-meshheading:10064084-H(+)-K(+)-Exchanging ATPase,
pubmed-meshheading:10064084-Mice,
pubmed-meshheading:10064084-Mice, Inbred BALB C,
pubmed-meshheading:10064084-Th1 Cells,
pubmed-meshheading:10064084-Th2 Cells,
pubmed-meshheading:10064084-Thymectomy
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pubmed:year |
1999
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pubmed:articleTitle |
Post-thymectomy autoimmune gastritis: fine specificity and pathogenicity of anti-H/K ATPase-reactive T cells.
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pubmed:affiliation |
Cellular Immunology Section, Laboratory of Immunology, NIAID, NIH, Bethesda 20892-1892, USA.
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pubmed:publicationType |
Journal Article
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